Plant Diversity ›› 2007, Vol. 29 ›› Issue (04): 388-392.

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Assessment of Genetic Diversity of Paris polyphylla (Trilliaceae) by ISSR Markers

HE Jun1 , 2 , YANG Bai-Yun1 , CHEN Shao-Feng1 , GAO Lian-Ming2 , WANG Hong2   

  1. 1 Colleage of Life Science , Nanchang university, Nanchang 330047 , China; 2 Laboratory of biodiversity and biogeography ,
    Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 , China
  • Received:2006-10-09 Online:2007-08-25 Published:2007-08-25
  • Contact: WANG Hong

Abstract: Some species of the genus Paris are of high medicinal value . Severe human activities particularly over-harvesting have led to recent declines of their population size and fragmented habitat patches . Inter-simple sequence repeat markers ( ISSR) were applied to investigate the genetic diversity of eight populations of Paris polyphylla in Yunnan and Sichuan Provinces. Fourteen primers resulted to a total of 251 bands from the eight populations, of which 235 were polymorphic, which showes a high level of genetic diversity in this species . A relatively high level of genetic diversity was revealed: PPB
= 93 . 63% , HE = 0 . 2204 and HO = 0. 3532 at the species level; PPB = 50. 45%, HE = 0.1405 and HO = 0.2194 at the population level. The GST= 0. 3625 indicated a high degree of genetic differentiation among populations . Genetic variation occurred mainly within populations . In UPGMA cluster analysis the six populations of P. polyphylla var. yunnanensis were divided into two clades , and P. polyphylla var. yunnanensis is more related to P. polyphylla var . stenophylla than to P. polyphylla var . pseudothibetica . In this paper , some conservation concerns were discussed which would be helpful for the conservation and sustainable utilization of this medicine resources.

Key words: Paris polyphylla

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