Plant Diversity ›› 2007, Vol. 29 ›› Issue (04): 444-446.

• Articles • Previous Articles     Next Articles

Production of Flavonoids in Cell Suspension Culture of Glycyrrhiza inflata (Leguminosae)

YANG Ying, HE Feng , YU Long-Jiang, CHEN Xue-Hong, LEI Jing, JI Jia-Xing, FU Chun-Hua   

  1. College of Life Science and Technology , Huazhong University of Science and Technology , Wuhan 430074 , China
  • Received:2006-12-05 Online:2007-08-25 Published:2007-08-25
  • Contact: YU Long-Jiang

Abstract: The cell biomass, flavonoids production and nutrients consumption were monitored to characterize the culture of progressive scale-up of Glycyrrhiza inflata . The maximum biomass, flavonoids production and the maximum specific growth rate in the culture of 250 and 500mL flasks remain similar , but were significantly higher than that of 1 000 mL flask. The three parameters in the culture of 1 000mL flask were 27% , 30% , and 27% lower than that of 250 mL flask , respectively. The concentrates of phosphate , nitrate and ammonium in progressive scale-up flasks decreased similarly . Phosphate and nitrate were almost exhausted on day 10 and 18 in all flasks, respectively; while ammonium maintained about 100mg/L till cells were harvested. The basic culture conditions needed to be further optimized for higher flavonoids production on a bioreactor scale .

Key words: Glycyrrhiza inflata

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