Abstract: This paper studied on 28 pepper materials, including 16 cultivated papper materials, 3 wild pepper materials, 2 wild relatives of pepper materials , 6 kava ( Piper methysticum) materials, and 1 Peperomia pellucida materials. According to RAPD analysis, we generate SCAR marker for identifying Kava ( Piper, Piperaceae ) . A Kava-associated fragment with a length ofabout 400 bp was generated with OPQ-03 primer . The fragment was cloned and sequenced . PCR amplification with the specific primers P7 . 1 ( 5′-GGT CAC CTC ACC GCA GCA GGA TGA ACG-3′) and P7 .2 (5′-GGT CAC CTC AAT GAC ATG GGA TGA ATC-3′) was performed to 28 materials , which 27 materials amplified the 440 bp specific band except for Peperomia pellucida Kunth .

Key words: Piper nigrum