Abstract: Nutrient solution was added into soil to improve the growth of hyphae and its host seedlings ( Coffea arabica). Pure culture of Phlebopus portentosus was inoculated in the roots of the host seedling. Results showed that over 92.5% of seedlings were found with mycorrhiza . External hyphae develop to the top or side of the roots, and even in the soil around roots. Fungal colonies (shiros) were ultimately formed. It was found that young fruit bodies appeared around or among the host seedlings after inoculation of 20 days. 52 of 162 young fruit bodies grew into mature in pots.

Key words: Phlebopus portentosus