Plant Diversity ›› 2007, Vol. 29 ›› Issue (05): 559-562.

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Cultivation and Molecular Identification of Two Bolete Strains Isolated from Boletus edulis Complex of Yunnan, China

WANG Hai-Kun1, 2, LI Tao1, ZHAO Dan-Dan3, LI Ling-Fei1, SHA Tao1, ZHAO Zhi-Wei1


  1. 1 Key Laboratory for Conservation and Utilization of Bio-Resources, and Key Laboratory for Microbial Resources of Minitry of Education , Yunnan University , Kunming 650091 , China ; 2 Kunming Teachers College, Kunming 650031 , China; 3 Institute of Horticulture of Yunnan Academy of Agricultural Sciences, Kunming 650205 , China

  • Received:2007-03-05 Online:2007-10-25 Published:2007-10-25
  • Contact: ZHAO Zhi-Wei

Abstract: Two bolete strains were obtained from fruiting bodies by tissue isolation and culture methods. Conditions for isolation and cultivation of the two strains were studied .Molecular identification was carried out based on sequence analysis of the internal transcribed spacers ( ITS1-5.8S-ITS2) of the strains. Neighbour-joining analysis showed that the two isolates belong to the Boletus edulis complex and have a close phylogenetic relationship to B. aestivalis.

Key words: Boletus edulis

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