Abstract: We have developed a reproducible method of small RNAs cloning from model plant Arabidopsis thaliana , which will facilitate the research on small RNAs in the future .We have successfully cloned several small RNAs from leaves of Arabidopsis treated by SA ( signal molecule of disease resistance ) , using isolation , adapter ligation , PCR amplification , Tclone, sequence and bio-information analysis and so on . Furthermore , we conducted analysis on their expression profiles and predicted functions of these small RNAs .

Key words: Arabidopsis