Plant Diversity ›› 2009, Vol. 31 ›› Issue (S16): 29-36.

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Successful Mycorrhizal Synthesis of Tuber indicum with Two Indigenous Hosts and Their Recognition

GENG Li-Ying1 , WANG Xiang-Hua1 , YU Fu-Qiang1 , DENG Xiao-Juan1 , 2 ,
SHI Xiao-Fei1 , 2 , XIE Xue-Dan1 , 2 , LIU Pei-Gui1

  1. 1 Key Laboratory of Biodiversity & Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 , China ; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China
  • Online:2009-12-25 Published:2009-12-25

Abstract: Tuber indicum is one of the most renowned commercialized fungi in China. Mycorrhizal investigations, however, have been carried out mainly with exotic trees . Up to now there is no detailed description of morphology of the mycorrhizae formed with the indigenous hosts of T. indicum . Containerized seedlings of two indigenous hosts of the fungus in southwestern China, Pinus armandii and Castanea mollissima were inoculated with aqueous spore suspension of T. indicum in two kinds of substrates .Mycorrhizae began to form4 months after inoculation and were harvested at 9 months. The contributing fungus of the mycorrhizae was confirmed to be T. indicum by morphological and ITS-rDNA sequence analyses. The morphology of emanating hyphae and epidermoid-like mantle appearance was similar to the mycorrhizae obtained with some European trees. The high morphological variation and the similarity to that of T. melanosporum makes it difficult to distinguish the mycorrhizae of the two species bymorphology alone . The synthesis and their recognition of mycorrhizae of T. indicum with its indigenous hosts will be of great significance for planned cultivation of the Asian black truffles .

Key words: Asian black truffle

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