Interaction between Endophytic Fungi and Seedlings of Two Species of Paphiopedilum during Symbiotic Culture

doi:10.3724/SP.J.1143.2012.11144

Abstract

Abstract:

Two fungi PM1 and PA1 were isolated from P.micranthum and P.armeniacum, respectively, the former belonged to Epulorhiza while the latter belonged to Tulasnella calospora. In this study, seedlings of P.armeniacum and P.micranthum were inoculated with PM1 on DE medium, the biomass and the content of Ca, Mn of both P.armeniacum and P.micranthum seedlings were higher than those in the control seedlings, which indicated that PM1 could improve the growth of those species. Seedlings of P.armeniacum and P.micranthum were inoculated with PA1 on DE medium, the biomass in both species was no difference with those in the control ones. But PA1 enhanced the dry weight of P.armeniacum according to the rate of dry weight increase. The impact of PM1 on P.micranthum was higher than on P.armeniacum, while PA1 impacted conversely, which demonstrated that fungi had stronger improving effect only on its own host plants instead of any other orchids. P.armeniacum seedlings were inoculated with PM1 and PA1 on Harvais medium. The growth of seedlings was inhibited by fungi. The results indicated that nutrient level of medium played an important role in affecting the symbiotic relationship between P.armeniacum seedings and endophytic fungi. Poornutrient medium was beneficial to the symbiotic relationship and nutrientrich medium was not conducive to the symbiosis.

Key words: Paphiopedilum micranthum, P.armeniacum, Endophytic fungi, Epulorhiza, Tulasnella calospora, Symbiosis, Interaction

CLC Number:

SHU Xin-Min-, HU Hong. Interaction between Endophytic Fungi and Seedlings of Two Species of Paphiopedilum during Symbiotic Culture[J]. Plant Diversity, 2012, 34(2): 171-178.

References

陈心启, 吉占和, 1998. 中国兰花全书［M］. 北京: 中国林业出版社, 118
Athipunyakom P, Manoch L, Piluek C, 2004. Isolation and identification of mycorrhizal fungi from eleven terrestrial orchids［J］. Kasetsart Journal (Natural Science), 38: 216—228
Cameron DD, Johnson I, Leake JR et al., 2007. Mycorrhizal acquisition of inorganic phosphorus by the greenleaved terrestrial orchid Goodyera repens［J］. Annals of Botany, 99: 831—834
Chutima R, Dell B, Vessabutr S et al., 2011. Endophytic fungi from Pecteilis susannae (L.) Rafin (Orchidaceae), a threatened terrestrial orchid in Thailand［J］. Symbiosis, 53: 149—156
Dearnaley JDW, 2007. Further advances in orchid mycorrhizal research［J］. Mycorrhiza, 17(6): 475—486
Dijk E, Eck ND, 1995. Effects of mycorrhizal fungi on in vitro nitrogen response of some Dutch indigenous orchid species［J］. Canadian Journal of Botany, 73: 1203—1211
Dong YL (董艳莉), Yan H (阎洪), Guo QS (郭泉水) et al., 2008. The natural distribution investigation and cultivation trial of Paphiopedilum armeniacum［J］. Forest Research (林业研究), 21 (1): 25—30
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］. Phytochem Bull, 19: 11—15
Hadley G, 1970. Nonspecificity of symbotic infection in orchid mycorrhiza［J］. New Phytologist, 69: 1015—1023
Harvais G, 1982. An improved culture medium for growing the orchid Cypripedium reginae axenically［J］. Canadian Journal of Botany, 60: 2547—2555
Jin H (金辉), Xu ZX (许忠祥), Chen JH (陈金花) et al., 2009. Interaction between tissuecultured seedlings of Dendrobium officinale and mycorrhizal fungus (Epulorhiza sp.) during symbiotic culture［J］. Chinese Journal of Plant Ecology (植物生态学报), 33 (3): 433—441
Long B (龙波), Long CL (龙春林), 2006. Amazing Paphiopedilum and its research status［J］. Chinese Journal of Nature (自然杂志), 28 (6): 341—344
Luo YB (罗毅波), Jia JS (贾建生), Wang CL (王春铃), 2003. Conservation strategy and potential advantages of the Chinese Paphiopedilum［J］. Biodiversity Science (生物多样性), 11 (6): 491—498
Nontachaiyapoom S, Sasirat S, Manoch L, 2010. Isolation and identification of Rhizoctonialike fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand［J］. Mycorrhiza, 20: 459—471
Otero JT, Ackerman JD, Bayman P, 2002. Diversity and host specificity of endophytic Rhizoctonialike fungi from tropical orchids［J］. American Journal of Botany, 89 (11): 1852—1858
Otero JT, Ackerman JD, Bayman P, 2004. Differences in mycorrhizal preferences between two tropical orchids［J］. Molecular Ecology, 13: 2393—2404
PorrasAlfaro A, Bayman P, 2007. Mycorrhizal fungi of Vanilla: diversity, specificity and effects on seed germination and plant growth［J］. Mycologia, 99: 510—525
Rasmussen HN, Rasmussen FN, 2007. Trophic relationships in orchid mycorrhizadiversity and implications for conservation［J］. Lankesteriana, 7: 334—341Shefferson RP, Kull T, Tali K, 2008. Mycorrhizal interactions of orchids colonizing Estonian mine tailings hills［J］. Amercian Journal of Botany, 95: 156—164Taylor DL, Bruns TD, Szaro TM et al., 2003. Divergence in mycorrhizal specialization within Hexalectris spicata (Orchidaceae), a nonphotosynthetic desert orchid［J］. Amercia Journal of Botany, 90: 1168—1179Taylor DL, Bruns TD, Hodges SA, 2004. Evidence for mycorrhizal races in a cheating orchids［J］. Proceedings the Royal Society Lond B, 271: 35—43White TJ, Bruns T, Lee S et al., 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics［M］. Academic Press, 315—322Yu XM (于雪梅), Guo SX (郭顺星), 2000. Establishment of symbiotic system for Anoectochilus roxburghii (Wall) Lindl. and endophytic fungi［J］. China Journal of Chinese Materia Medica (中国中药杂志), 25 (2): 81—83Yuan L, Yang ZL, Li SY et al., 2010. Mycorrhizal specificity, preference, and plasticity of six slipper orchids from South Western China［J］. Mycorrhiza, 20 (8): 559—568Zettler LW, Poulter SB, McDonald KI et al., 2007. Conservationdriven propagation of an epiphytic orchid (Epidendrum nocturnum) with a mycorrhizal fungus［J］. HortScience, 42: 135—136Zhao YJ (赵杨景), Guo SX (郭顺星), Gao WW (高薇薇) et al., 1999. The symbiosis of three endophytic fungi with Cymbidium sp. and its effects on the mineral nutrition absorption［J］. Acta Horticulturae Sinica (园艺学报), 26 (2): 110—115

[1]	Jin Zhao, Yuanjie Li, Xuanni Wang, Manru Li, Wenbin Yu, Jin Chen, Ling Zhang. Parasite-host network analysis provides insights into the evolution of two mistletoe lineages (Loranthaceae and Santalaceae) [J]. Plant Diversity, 2023, 45(06): 702-711.
[2]	Yanliang Wang, Xinhua He, Fuqiang Yu. Non-host plants: Are they mycorrhizal networks players? [J]. Plant Diversity, 2022, 44(02): 127-134.
[3]	Juan Song, Jinge Bian, Na Xue, Yuxing Xu, Jianqiang Wu. Inter-species mRNA transfer among green peach aphids, dodder parasites, and cucumber host plants [J]. Plant Diversity, 2022, 44(01): 1-10.
[4]	Yunhua Wang, Nan Li, Ting Chen, Yiqing Gong. Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis [J]. Plant Diversity, 2018, 40(05): 245-252.
[5]	Xinhui Li, Lin Liu, Xu Gu, Jianying Xiang. Heavy collecting induces smaller and deeper Fritillariae Cirrhosae Bulbus in the wild [J]. Plant Diversity, 2017, 39(04): 208-213.
[6]	Tao Liu, Alex Greenslade, Shengchao Yang. Levels of rhizome endophytic fungi fluctuate in Paris polyphylla var. yunnanensis as plants age [J]. Plant Diversity, 2017, 39(01): 60-64.
[7]	CHEN Shan-, REN Zong-Xin-, WANG Hong-, Wu Ding. The Classification and Functional Significance of Staminodes in Angiosperms [J]. Plant Diversity, 2015, 37(06): 683-692.
[8]	HUANG Jia-Lin, LI Shu-Yun, HU Hong. ISSR and SRAP Markers Reveal Genetic Diversity and Population Structure of an Endangered Slipper Orchid, Paphiopedilum micranthum (Orchidaceae) [J]. Plant Diversity, 2014, 36(02): 209-218.
[9]	JIN Xin, Yang-Ming-Zhi, LIU Fei-Hu. Effects of Endophytic Fungi Reinoculation on Physiological and Agronomic Characters of Hemp (Cannabis sativa) [J]. Plant Diversity, 2014, 36(01): 65-69.
[10]	DENG Zhi-Jun ^, , SONG Song-Quan. Inhibitory Effect of ABA on Seed Germination of Dalbergia fusca (Leguminosae) and Antagonism of Other Phytohormones to ABA [J]. Plant Diversity, 2008, 30(04): 440-446.
[11]	JIANG Xi-Bing ^, , YU Di-Qiu. Peptide Hormones in Plants [J]. Plant Diversity, 2008, 30(03): 333-339.
[12]	LIU Jing-Xin^{, ,} CHEN Jin. Preliminary Investigation of Extrafloral Nectaried Plants the Tropical Rainforests in Xishuangbanna, China [J]. Plant Diversity, 2008, 30(02): 173-182.
[13]	GUO Hui - Jun LI Heng DAO Zhi - Ling. Dynamism of Socio - Economy and Biodiversity Interaction - A Case from Gaoligong Mountains [J]. Plant Diversity, 2000, 22(12): 1-3.
[14]	Luo Hongyuan, Gao Xiang, Peng Zhiliang, Hu Zhongmei. STUDIES ON THE HEREDITY OF TRANSFOMING DOMINANT INTERACTION OF NUCLAR GENE FROM BRASSICA PEKINENSIS TO B.CAMPESTRIS [J]. Plant Diversity, 1997, 19(01): 1-3.