Plant Diversity ›› 2014, Vol. 36 ›› Issue (01): 29-36.

• Articles • Previous Articles     Next Articles

Diversity of Culturable Bacteria Associated with Ascocarps of a Chinese White Truffle, Tuber panzhihuanense (Ascomycota)*

 WANG  Shan-Ping-1、2, LIU  Pei-Gui-1   

  1. 1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2014-01-25 Published:2014-12-01


Culturable bacterial communities inhabiting ascocarps of Tuber panzhihuanense were investigated. Isolates obtained on tryptone soy agar (TSA) were screened with high performance capillary electrophoresis (HPCE) according to differences in size of 16S rDNA V3. Target isolates were identified by analysis of the whole length of 16S rDNA gene. The results revealed that the ascocarps of T. panzhihuanense harbored a great number of culturable bacteria which belonging to 20 species and 11 genera in 5 phyla. Most isolates (4968%) were affiliated to the γProteobacteria, dominated by Pseudomonas lurida. The second major subclass was αProteobacteria (3742%), with Phyllobacterium and a nitrogenfixing bacterium Bradyrhizobium japonicum also occurring as dominant taxa. The remaining bacterial isolates contained members of Actinobacteria (322%) and Firmicutes (774%) of which Bacillus was the commonest bacterium. A novel Tuberassociated culturable bacterium species, Terriglobus roseus, was isolated and detected for the first time in Tuber ascocarps.

Key words: Tuber panzhihuanense, Ascocarp, Bacteria diversity, 16S rDNA

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