Mycorrhizal Fungi Promote Growth and Nitrogen Utilization by Dendrobium nobile (Orchidaceae)

doi:10.7677/ynzwyj201413117

Abstract

Abstract:

Mycorrhizal associations play a key role in the life cycle and evolutionary history of orchids. Although most orchid species are tropical and epiphytic, their mycorrhizae are poorly understood compared with those of temperate, terrestrial orchids. To investigate the influences of such fungi on photosynthetic, epiphytic orchids, we inoculated seedlings of Dendrobium nobile with Epulorhiza sp. (S1) or Tulasnella sp. (S3). These fungi had been identified based on their morphological and molecular characters. Both S1 and S3 formed symbiotic associations with our seedlings, promoting their growth and development to various degrees. Results from signature experiments with the 15N stable isotope suggested that the utilization of organic nitrogen by orchid seedlings was significantly improved by S1, but not by S3. Dendrobine contents were significantly higher in all inoculated seedlings. Our findings demonstrate that these mycorrhizal fungi enhance plant growth, their utilization of organic nitrogen, and the accumulation of secondary metabolites in this epiphytic orchid species.

Key words: Dendrobium nobile, Mycorrhizal fungi, Growth, 15N, Dendrobine

CLC Number:

Q 945
Q 948

WANG Qiu-Xia, YAN Ning, JI Da-Gan, LI Shu-Yun, HU Jiang-Miao, HU Hong. Mycorrhizal Fungi Promote Growth and Nitrogen Utilization by Dendrobium nobile (Orchidaceae)[J]. Plant Diversity, 2014, 36(03): 321-330.

References

Altschul SF, Gish W, Miller W et al., 1990. Basic local alignment search tool［J］. Journal of Molecular Biology, 215: 403—410
Bayman P, González EJ, Fumero JJ et al., 2002. Are fungi necessary? How fungicides affect growth and survival of the orchid Lepanthes rupestris in the field［J］. Journal of Ecology, 90: 1002—1008
Bidartondo MI, Bruns TD, Wei M et al., 2003. Specialized cheating of the ectomycorrhizal symbiosis by an epiparasitic liverwort［J］. Proceedings of the Royal Society of London Series BBiological Sciences, 270: 835—842
Bruns TD, Szaro TM, Gardes M et al., 1998. A sequence database for the identification of ectomycorrhizal basidiomycetes by phylogenetic analysis［J］. Molecular Ecology, 7: 257—272
Cameron DD, Leake JR, Read DJ, 2006. Mutualistic mycorrhiza in orchids: evidence from plantfungus carbon and nitrogen transfers in the greenleaved terrestrial orchid Goodyera repens［J］. New Phytologist, 171: 405—416
Cechin I, Fumis TF, 2004. Effect of nitrogen supply on growth and photosynthesis of sunflower plants grown in the greenhouse［J］. Plant Science, 166: 1379—1385
Chen XM (陈晓梅), Guo SX (郭顺星), 2005. Effects of four species of endophytic fungi on the growth and polysaccharides and alkaloid contents of Dendrobium nobile［J］. China Journal of Chinese Materia Medica (中国中药杂志), 30(4): 253—257
Chutima R, Dell B, Lumyong S, 2011. Effects of mycorrhizal fungi on symbiotic seed germination of Pecteilis susannae (L.) Rafin (Orchidacheae), a terrestrial orchid in Thailand［J］. Symbiosis, 53: 149—156
Dearnaley JDW, 2007. Further advances in orchid mycorrhizal research［J］. Mycorrhiza, 17: 475—486
Doehlemann G, Wahl R, Vranes M et al., 2008. Establishment of compatibility in the Ustilago maydis/maize pathosystem［J］. Journal of Plant Physiology, 165: 29—40
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］. Phytochemistry Bulletin, 19: 11—15
Gebauer G, Meyer M, 2003.15N and 13C natural abundance of autotrophic and mycoheterotrophic orchids provides insight into nitrogen and carbon gain from fungal association［J］. New Phytologist, 160: 209—223
Haslam E, 1986. Secondary metabolismfact and fiction［J］. Natural Product Reports, 3: 217—249
Hou XQ, Guo SX, 2009. Interaction between a dark septate endophytic isolate from Dendrobium sp. and roots of Dnobile seedlings［J］. Journal of Integrative Plant Biology, 51: 374—381
Johnson ND, Liu B, Bentley BL, 1987. The effects of nitrogen fixation, soil nitrate, and defoliation on the growth, alkaloids, and nitrogen levels of Lupinus succulentus (Fabaceae) ［J］. Oecologia, 74: 425—431
Jones DL, Healey JR, Willett VB et al., 2005. Dissolved organic nitrogen uptake by plantsan important N uptake pathway?［J］. Soil Biology and Biochemistry, 37: 413—427
Kristiansen KA, Taylor DL, Kjller R et al., 2001. Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis (Orchidaceae) using singlestrand conformation polymorphism and mitochondrial ribosomal large subunit DNA sequences［J］. Molecular Ecology, 10: 2089—2093
Lawlor DW, Kontturi M, Young AT, 1989. Photosynthesis by flag leaves of wheat in relation to protein, ribulose bisphosphate carboxylase activity and nitrogen supply［J］. Journal of Experimental Botany, 40: 43—52
Li S(李墅), Wang CL (王春兰), Guo SX (郭顺星), 2009. Determination of dendrobine in Dendrobium nobile by HPLC analysis［J］. Chinese Pharmaceutical Journal (中国药学杂志), 4: 252—254
Liebel HT, Gebauer G, 2011. Stable isotope signatures confirm carbon and nitrogen gain through ectomycorrhizas in the ghost orchid Epipogium aphyllum Swartz［J］. Plant Biology, 13: 270—275
Liu HX, Luo YB, Liu H, 2010. Studies of mycorrhizal fungal of Chinese orchids and their role in orchid conservation in China—A Review［J］. Botany Review, 76: 241—262
Ma M, Tan TK, Wong SM, 2003. Identification and molecular phylogeny of Epulorhiza isolates from tropical orchids［J］. Mycology Research, 107: 1041—1049
McCormick MK, Whigham DF, O’Neill J, 2004. Mycorrhizal diversity in photosynthetic terrestrial orchids［J］. New Phytologist, 163: 425—438
Midgley DJ, Jordan LA, Saleeba JA et al., 2006. Utilisation of carbon substrates by orchid and ericoid mycorrhizal fungi from Australian dry sclerophyll forests［J］. Mycorrhiza, 16: 175—182
Mohanty P, Das MC, Kumaria S et al., 2012. Highefficiency cryopreservation of the medicinal orchid Dendrobium nobile Lindl［J］. Plant Cell, Tissue and Organ Culture, 109: 297—305
Nontachaiyapoom S, Sasirat S, Manoch L, 2010. Isolation and identification of Rhizoctonialike fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand［J］. Mycorrhiza, 20: 459—471
Nontachaiyapoom S, Sasirat S, Manoch L, 2011. Symbiotic seed germination of Grammatophyllum speciosum Blume and Dendrobium draconis Rchb. f., native orchids of Thailand［J］. Scientia Horticulturae, 130: 303—308
PorrasAlfaro A, Bayman P, 2007. Mycorrhizal fungi of Vanilla: diversity, specificity and effects on seed germination and plant growth［J］. Mycologia, 99: 510—525
Rasmussen HN, 2002. Recent developments in the study of orchid mycorrhiza［J］. Plant and Soil, 244: 149—163
Scheible WR, Morcuende R, Czechowski T et al., 2004. Genomewide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen［J］. Plant Physiology, 136: 2483—2499
Song JY (宋经元), Guo SX (郭顺星), 2001. Effects of fungus on the growth of Dendrobium candium and Dnobile in vitro culture［J］. Acta Academiae Medicinae Sinicae (中国医学科学院学报), 23: 547—551
Suárez JP, Weiβ M, Abele A et al., 2006. Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest［J］. Mycological Research, 110: 1257—1270
Yuan L, Yang ZL, Li SY et al., 2010. Mycorrhizal specificity, preference and plasticity of six slipper orchids from South Western China［J］. Mycorrhiza, 20: 559—568
Zha XQ, Luo JP, Jiang ST et al., 2007. Enhancement of polysaccharides production in suspension cultures of protocormlike bodies from Dendrobium huoshanense by optimization of medium compositions and feeding of sucrose［J］. Process Biochemistry, 42: 344—351

[1]	Cindy Q. Tang, Shi-Qian Yao, Peng-Bin Han, Jian-Ran Wen, Shuaifeng Li, Ming-Chun Peng, Chong-Yun Wang, Tetsuya Matsui, Yong-Ping Li, Shan Lu, Yuan He. Forest characteristics, population structure and growth trends of threatened relict Pseudotsuga forrestii in China [J]. Plant Diversity, 2023, 45(04): 422-433.
[2]	Yu-Wen Zhang, Yu-Cen Shi, Shi-Bao Zhang. Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile [J]. Plant Diversity, 2023, 45(03): 326-336.
[3]	Jing Zhu, Lan Jiang, De-Huang Zhu, Cong Xing, Meng-Ran Jin, Jin-Fu Liu, Zhong-Sheng He. Forest gaps regulate seed germination rate and radicle growth of an endangered plant species in a subtropical natural forest [J]. Plant Diversity, 2022, 44(05): 445-454.
[4]	Meng-Jiao Fu, Hai-Yang Wu, Dong-Rui Jia, Bin Tian. Evolutionary history of a desert perennial Arnebia szechenyi (Boraginaceae): Intraspecific divergence, regional expansion and asymmetric gene flow [J]. Plant Diversity, 2021, 43(06): 462-471.
[5]	Mi-Cai Zhong, Xiao-Dong Jiang, Wei-Hua Cui, Jin-Yong Hu. Expansion and expression diversity of FAR1/FRS-like genes provides insights into flowering time regulation in roses [J]. Plant Diversity, 2021, 43(02): 173-179.
[6]	Jiao Qin, Wei Zhang, Shi-Bao Zhang, Ji-Hua Wang. Similar mycorrhizal fungal communities associated with epiphytic and lithophytic orchids of Coelogyne corymbosa [J]. Plant Diversity, 2020, 42(05): 362-369.
[7]	Xue-Zhao Wang, Xiao-Lin Sui, Yan-Yan Liu, Lei Xiang, Ting Zhang, Juan-Juan Fu, Ai-Rong Li, Pei-Zhi Yang. N-P fertilization did not reduce AMF abundance or diversity but alter AMF composition in an alpine grassland infested by a root hemiparasitic plant [J]. Plant Diversity, 2018, 40(03): 117-126.
[8]	Jianguo Chen, Yanbo Li, Yang Yang, Hang Sun. How cushion communities are maintained in alpine ecosystems:A review and case study on alpine cushion plant reproduction [J]. Plant Diversity, 2017, 39(04): 221-228.
[9]	HUANG Wei, HU Hong. Effect of Growth Temperature on the Activity of Cyclic Electron Flow in Tobacco Leaves [J]. Plant Diversity, 2015, 37(03): 283-292.
[10]	Li-Xia, GUO Zhen-Hua. A Pilot Study on Internode Elongation in a Paleotropical Bamboo, Dendrocalamus latiflorus (Poaceae: Bambusoideae) [J]. Plant Diversity, 2014, 36(01): 22-28.
[11]	ZHANG Shi-Bao. Biomass Partitioning Affects the Growth of Pinus Species from Different Elevations [J]. Plant Diversity, 2014, 36(01): 47-55.
[12]	WANG Chong-Yun-, CHEN Mei-Qing-, HE Zhao-Rong-, BANG Ming-Chun-, LI Qi-Yang-, OU Guang-Long-, LANG Xue-Dong-, DANG Cheng-Lin. A Note on a Scleromicrophyllous Evengreen Broadleaved ForestAssociation Olea ferruginea, Pistacia weinmannifolia in Lancang (Upper Mekong) River [J]. Plant Diversity, 2012, 34(01): 81-88.
[13]	XIAO Jia-Xin-, REN Qun-, Wu Xue-Jun-, CHEN Ying-Ying-, ZHANG Shao-Ling. Effects of Arbuscular Mycorrhizal Fungi on Physiological Character of Bahia Grass (Paspalum notatum, Poaceae) [J]. Plant Diversity, 2011, 33(5): 521-528.
[14]	CHEN Guang-Li-, ZHOU De-Qun-, YANG Yong-Ping-, YANG Xue-Fei. Fruiting Pattern of Tricholoma matsutake and Its Relationship with Meteorological Factors in Yunnan, China [J]. Plant Diversity, 2011, 33(5): 547-555.
[15]	LANG Rong-, XIU Jian-Chu-, Timm Tennigkeit, YANG Xue-Fei-, BI Ying-Feng. A Study of Stand Growth Model for Pinus yunnanensis (Pinaceae) Based on Plots Data——A Case Study in Yangliu Township, Baoshan, Yunnan Province [J]. Plant Diversity, 2011, 33(3): 357-363.