Plant Diversity ›› 2020, Vol. 42 ›› Issue (02): 102-110.DOI: 10.1016/j.pld.2019.11.002

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Analysis of changes in the Panax notoginseng glycerolipidome in response to long-term chilling and heat

Tao Liua, Jia Chenb, Furong Xuc, Xiahong Hea, Shengchao Yanga, Youyong Zhua, Weiqi Lid, Guowei Zhengc   

  1. a National & Local Joint Engineering Research Center on Germplasm Utilization & Innovation of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, China;
    b Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, China;
    c College of Chinese Material Medica, Yunnan University of Chinese Medicine, Kunming, China;
    d Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
  • Received:2019-08-31 Revised:2019-11-21 Online:2020-04-25 Published:2020-04-30
  • Supported by:
    This work was supported by grants from National Natural Science Foundation of China (31560085, 81460581 and 31600215), High Level Talents Project of Yunnan University of Chinese Medicine (2019YZG07), Natural Science Fund of Yunnan Province (2017FG001), Yunnan Applied Basic Research Project (2016FA042, 2017FB057 and 2015FB171), Innovation Guidance and Scientific and Technological Enterprise Cultivation Plan in Yunnan Province (2017RA001). We thank Kansas Lipidomics Research Center for performing the lipidomic analysis.

Abstract: Long-term moderately high or low temperatures can damage economically important plants. In the present study, we treated Panax notoginseng, an important traditional Chinese medicine, with temperatures of 10, 20, and 30℃ for 30 days. We then investigated P. notoginseng glycerolipidome responses to these moderate temperature stresses using an ESI/MS-MS-based lipidomic approach. Both long-term chilling (LTC, 10℃) and long-term heat (LTH, 30℃) decreased photo pigment levels and photosynthetic rate. LTH-induced degradation of photo pigments and glycerolipids may further cause the decline of photosynthesis and thereafter the senescence of leaves. LTC-induced photosynthesis decline is attributed to the degradation of photosynthetic pigments rather than the degradation of chloroplastidic lipids. P. notoginseng has an especially high level of lysophosphatidylglycerol, which may indicate that either P. notoginseng phospholipase A acts in a special manner on phosphatidylglycerol (PG), or that phospholipase B acts. The ratio of sulfoquinovosyldiacylglycerol (SQDG) to PG increased significantly after LTC treatment, which may indicate that SQDG partially substitutes for PG. After LTC treatment, the increase in the degree of unsaturation of plastidic lipids was less than that of extraplastidic lipids, and the increase in the unsaturation of PG was the largest among the ten lipid classes tested. These results indicate that increasing the level of unsaturated PG may play a special role in maintaining the function and stability of P. notoginseng photosystems after LTC treatment.

Key words: Extraplastidic lipids, Glycerolipidome, Long-term chilling stress, Long-term heat stress, Panax notoginseng, Plastidic lipids