Plant Diversity ›› 2008, Vol. 30 ›› Issue (05): 570-576.

• Articles • Previous Articles     Next Articles

Clone and Function Prediction of Six Novel Small RNAs in Arabidopsis (Cruciferae)

TANG Fei 1, 2 , LIANG Gang1 , 2, YU Di-Qiu1   

  1. 1 Xishuangbanna Tropical Botanical Garden , Chinese Academy of Sciences, Kunming 650223 , China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China
  • Received:2008-01-09 Online:2008-10-25 Published:2008-10-25

Abstract: We have developed a reproducible method of small RNAs cloning from model plant Arabidopsis thaliana , which will facilitate the research on small RNAs in the future .We have successfully cloned several small RNAs from leaves of Arabidopsis treated by SA ( signal molecule of disease resistance ) , using isolation , adapter ligation , PCR amplification , Tclone, sequence and bio-information analysis and so on . Furthermore , we conducted analysis on their expression profiles and predicted functions of these small RNAs .

Key words: Arabidopsis

CLC Number: