Plant Diversity ›› 1998, Vol. 20 ›› Issue (03): 1-3.

• Articles •    

Cloning and Sequencing of a Part -Length cDNA Coding for Glycerol - 3 - Phosphate Acyltransferase from Rice

LIU Ji-Mei,CHEN Shan-Na,YAN Bo, HUANG Xing-Qi, YANG Ming-Zhi   

  1. Biology Department, Yunnan University , Kunming 650091;Institute of Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223
  • Online:1998-06-25 Published:1998-06-25

Abstract: According to the conserved ammo acid sequences of glycerol- 3- phosphate acyltransferase from several kinds of dicotyls, we designed and synthesized a pair of degenerate primers. RNA was isolated from the highly chilling- resistant rice "Ugeng No.2". A 315bp cDNA fragment was obtained with RT- PCR tech-nique. After purification, the cDNA fragment was cloned into pGEM - T vector directly. The result of identifica-tion by PCR method indicates that the recombinant plasmid pGTL5 contains a 315bp cDNA fragment. The result demonstrated that we had cloned a part of cDNA encoding glycerol - 3 - phosphate acyltransferase. While the cDNA sequence and deduced amino acid sequence reported here was compared with that published abroad, the identity was above 70%. This result hints that the gene is highly conservative on evolution.

Key words: Glycerol- 3 - phosphate acyltransferase (GPAT)