Plant Diversity ›› 1988, Vol. 10 ›› Issue (02): 1-3.

• Articles •    

THE GLUCOSINOLATES AND THIOGLUCOSIDASE OF CAPPARIS MASAIKAI SEEDS

Hu Zhong   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
  • Online:1988-04-25 Published:1988-04-25

Abstract: A new glucosinolate, the 2-hydroxyethylglucosinolate was isolated and identified from seeds of Capparis masaikai Levl, with a yield of 3% of dry seed meal. Its enzymatic product at pH 6.0 was oxazolidine- 2 -thione. The thioglucosidase was extracted from seed meal powder with 1 N Nad, precipitated with ammonium sulfate, and futher purified by gel filtration on Sephadex G50 and being passed through a CM-cellulose column. The purified thioglucosidase was showed to be of Mr =116 kD, pI =5.05, consisted of two near equal subunits. It differs from the thioglucosidases isolated from the seeds of Cruciferae, which are glyco proteins with Mr = 140 kD and a carbohydrate content of 15%, in that it was showed not to contain carbohydrate.

Key words: Capparis masaikai