Abstract: The hypocotyl of D.sophia was cultured to study the effect of some factors on the rate of bud regeneration.The results showed that the rate of hypocotyl differentiating was improved via a preculture of five-day period with the medium containing 2,4-D;AgNO3(1.7mg/L)could greatly improve the differentiating rate of hypocotyl;the best hormone combination wqs 2.0mg/L6-BA+0.2mg/L NAA when 1.7mg/L AgNO3 was added to this medium; the hypocotyl of yillow seedlings was more sensitive to hormone than that of green seedlings,its differentiating rate could reach as high as 86.05%.When the regeneration buds were transferred 1/2 MS+0.5mg/L NAA+0.5mg/L IBA,the rate of regeneration roots reached 100%

Key words: Descurainia sophia