Plant Diversity ›› 2001, Vol. 23 ›› Issue (01): 1-3.

• Articles •    

Genetic Transformation by Ti Plasmid in Trichosanthes kirilowii

LEI He-Tian SONG Jing-Yuan QI Jian-Jun ZHANG Yin-Ling YANG Jun-Shan GUO Zhi-Gang   

  1. Institute of Medicinal Plant, Chinese Academy of Medical Science, Peking Union Medical College
  • Online:2001-02-25 Published:2001-02-25

Abstract: Transformed crown tissue cultures from Trichosanthes kirilowii Maxim. were established by Ti plasmid in C58 strains. The crown issues grew well in free- hormone MS medium . Nopaline was inspected in the crown gall and this data showed that T-DNA of Ti plasmid had been transformed successfully .The content of proteins in the tissue was about 130.6mg/g (fresh weight)which was inspected by compound-color methods and its TCN-protein was inspected by SDS-PAGE analysis. The study showed that the production of TCN proteins from crown tissue cultures may be developed in prospect.

Key words: Trichosanthes kirilowii