Plant Diversity ›› 2010, Vol. 32 ›› Issue (02): 147-150.DOI: 10.3724/SP.J.1143.2010.09204

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Isolation and Molecular Characterization of RALFLike Gene BcRALF from Brassica campestris ssp.chinensis var.parachinensis

LI YanYan1, 2, CAO JiaShu2   

  1. 1 School of Life Sciences, Fuyang Teachers College, Fuyang 236041, China; 2 Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University , Hangzhou 310029, China
  • Received:2009-10-14 Online:2010-04-25 Published:2010-06-29
  • Contact: CAO JiaShu

Abstract: The rapid alkalinization factor (RALF) gene is a new found plant polypeptide signal molecule, wide spreading in higher plants. In this study BcRALF was cloned from Brassica campestris sspchinensis Makino varparachinensis based on BcMF14 (GenBank accession number EF523516) from Bcampestris ssp. chinensis var. communis cv. Aijiaohuang. The sequence of this gene was 273 bp (GU086228) and was identified as a rapid alkalization factor gene according to its high identity with Boleracea var botrytis BoRALF1 and Arabidopsis thaliana RALFL9. Protein characteristics and sequence structure were predicted, and moreover, many bioactive sites were found. The results showed that the characteristics of the BcRALF protein consistent with its category as a peptide signal molecule.

Key words: Brassica campestris sspchinensis varparachinensis

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