Seed Storage Behavior of Formanodendron doichangensis (Fagaceae), a Rare and Endangered Plant

doi:10.3724/SP.J.1143.2011.11009

Abstract

Abstract:

The seed storage behavior of Formanodendron doichangensis (Fagaceae) was still unclear due to the effect of empty seeds to the germination experiment caused by high abortion. Therefore there was lack of a best storage method for conserve this rare and endangered plant. In the present paper, the Selecta Gravity Separator and the Xray imagine systems were introduced to successfully enhance the portion of the full and healthy seeds from the high aborted seed lot, and exclude the effect of empty seeds to the germination experiment. Moreover, the desiccation tolerance and low temperature tolerance of F.doichangensis seeds were tested to identify the seed storage behavior and the best storage method for this endangered species was provided. The results showed that Selecta Gravity Separator could efficiently improved the percentage of full seeds in the seed lot from 47% (before clean) to 86% (after clean). F.doichangensis seeds kept high viability at 95% total germination similar to that of control seeds (92%), when experienced the extreme desiccation treatment (moisture content below 5%). After the storage at low temperature (-20℃) and liquid nitrogen (-196℃) for three months with low moisture content (<5%), the seed germination remained at 90% and 91% respectively. In conclusion, the mature F.doichangensis seeds were desiccation tolerant and low temperature tolerant, which exhibited orthodox seed storage behavior according to the international seed storage behavior classification, and could be kept in the seed bank storage facilities for long term conservation.

Key words: Formanodendron doichangensis, Seed storage behavior, Desiccation tolerance, Low temperature tolerance

CLC Number:

Q 945

CHEN Hong-Ying-, SUN Wei-Bang-, LI Wei-Qi. Seed Storage Behavior of Formanodendron doichangensis (Fagaceae), a Rare and Endangered Plant[J]. Plant Diversity, 2011, 33(5): 540-546.

References

傅立国, 1992. 中国植物红皮书—稀有濒危植物 (第1册)［M］. 北京: 科学出版社, 302
Bonner FT, 1996. Responses to drying of recalcitrant seeds of Quercus nigra L.［J］. Annals of Botany, 78(2): 181—187
Chen GK (陈国科), 2005. A preliminary study of reproductive biology and population ecology of Sladenia celastrifolia Kurz. Kunming: Kunming Institute of Botany, Chinese Academy of Sciences (Master′s thesis), 30
Connor KF, Sowa S, 2003. Effects of desiccation on the physiology and biochemistry of Quercus alba acorns［J］. Tree Physiology, 23 (16): 1147—1152Dickie JB, Pritchard HW, 2002. Systematic and evolutionary aspects of desiccation tolerant in seeds［A］. In: Black M, Pritchard HW eds. Desiccation and Survival in Plants: Drying Without Dying［M］. London: CABI publishing, 239—259Ellis RH, 1973. Predicting the storage life of seeds［J］. Seed Science and Technology, 1: 499—514Ellis RH, Hong TD, Roberts EH, 1990. An intermediate category of seed sotrge behaviour? I. Coffee［J］. Journal of Experimental Botany, 41(9): 1167—1174
Ellis RH, MaiHong T, Hong TD et al., 2007. Comparative analysis by protocol and key of seed storage behaviour of sixty Vietnamese tree species［J］. Seed Science and Technology, 35 (2): 460—476
FinchSavage WE, Blake PS, Clay HA, 1996. Desiccation stress in recalcitrant Querus robber L. seeds results in lipid peroxidation and increased synthesis of jasmonates and abscisic acid［J］. Journal of Experimental Botany, 47 (5): 661—667
Gao H (高辉), Tang Y (唐亚), Zhang LY (张立芸), 2006. Megasporogenesis and embryo sac development in Excentrodendron hsienmu (Malvaceae) with reference to the systematic affinities of Excentrodendron［J］. Acta Phytotaxonomica Sinica (植物分类学报), 44 (5): 538—550
Hong TD, Ellis RH, 1996. A protocol to determine seed storage behaviour. IPGRI Technical Bulletin No.1［Z］. Rome: International Plant Genetic Resources Institute
ISTA, 2007. International Rules for Seed Testing［M］. edition 2008. Zürich, Switzerland: International Seed Testing Association
Leprince O, Buitink J, Hoekstra FA, 1999. Axes and cotyledons of recalcitrant seeds of Castanea sativa Mill. exhibit contrasing responses of respiration to drying in relation to desiccation sensitivity［J］. Journal of Experimental Botany, 50 (338): 1515—1524
Li DZ, Pritchard HW, 2009. The science and economics of ex situ plant conservation［J］. Trends in Plant Science, 14 (11): 614—621
Liu MS, Chang CY, Lin TP, 2006. Comparison of phospholipids and their fatty acids in recalcitrant and orthodox seeds［J］. Seed Science and Technology, 34 (2): 443—452
Nixon KC, Crepet WL, 1989. Trigonobalanus (Fagaceae): Taxonomic status and phylogenetic relationships［J］. American Journals of Botany, 76: 828—841
Pan YZ (潘跃芝), Gong X (龚洵), 2002. Megasporogenesis, development of female gametophyte of Manglietia insignis (Magnoliaceae)［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 22 (5): 1209—1214
Pence VC, 1990. Cryostorage of embryo axes of several largeseeded temperate tree species［J］. Cryobiology, 27: 212—218
Pritchard HW, 1990. Water potential and embryonic axis viability in recalcitrant seeds of Quercus rubra［J］. Annals of Botany, 67 (1): 43—49
Qin HZ (秦慧珍), Li BY (李碧媛), 1996. Effects of female gametophyte abortion on the reproduction of Liriodendron chinense populations［J］. Journal of Plant Resources and Enviornment (植物资源与环境), 5 (3): 1—5
Sun WB (孙卫邦), Zhou Y (周元), Zhao JC (赵金超) et al., 2004. Current distribution, population attributes and biological characters of Trigonobalanus doichangensis in relation to its conservation［J］. Acta Ecologica Sinica (生态学报), 24: 352—358
Tian MH, Tang AJ, 2010. Seed desiccation sensitivity of Quercus fabri and Castanopsis fissa (Fagaceae)［J］. Seed Science and Technology, 38 (1): 225—230
Xia K (夏柯), 2010. Fruit biology of genus Quercus (Fagaceae)［D］. Kunming Institute of Botany, 14—15
Xie GW (谢国文), Wen L (文林), 1999. Distribution status and conservation of Monimopetalum chinense ［J］. Biodiversity Science (生物多样性), 7 (1): 15—19
Xin X (辛霞), Lin J (林坚), Jing XM (景新明), 2007. Dehydration and chilling sensitivity of several Fagaceae seeds［J］. Seed (种子), 26 (3): 10—13Zheng YL (郑艳玲), 2010. Germplasm conservation and acclimatization biology of the endangered Trigonobalanus doichangensis (Fagaceae)［D］. Kunming Institute of Botany, 31—41Zhou Y (周元), Sun WB (孙卫邦), Li CR (李从仁), 2003. Preliminary Study on Germination of Trigonobalanus doichangensis［J］. Journal of Wuhan Botanical Research, (武汉植物研究), 21 (1): 73—76Zhou ZK (周浙昆), 1992. Origin, phylogeny and dispersal of Quercus from China［J］. Acta Botanica Yunnanica (云南植物研究), 14 (3): 227—236

[1]	YANG Ya-Juan, GUO Yong-Jie, QIN Shao-Fa, HU Xiao-Jian. Seed Storage Behavior and Seed Germination of Nine Species of Lauraceae from Yunnan, China [J]. Plant Diversity, 2015, 37(06): 813-820.
[2]	XUE Peng, WEN Bin. Effects of Drying Rates on the Desiccation Tolerance of Citrus maxima ‘Feizhouyou’ Seeds [J]. Plant Diversity, 2015, 37(03): 293-300.
[3]	JI MIng-Yue, ZHANG Nan, YAN Qi, WEN Bin. Storage Behavior Characteristics of Recalcitrant Podocarpus nagi Seeds* [J]. Plant Diversity, 2015, 37(01): 63-70.
[4]	ZHANG Li-Xia-, LAN Qin-Ying-, LI Hai-Tao-, TAN Yun-Hong-, GUAN Yan-Hong-, LI Xue-Lan. Developmental Changes in Relation to Desiccation Tolerance and Storage Characteristics of Aquilaria sinensis (Thymelaeaceae) Seeds [J]. Plant Diversity, 2011, 33(4): 458-464.
[5]	LUO Yin-Ling^,, SONG Song-Quan, LAN Qin-Ying. Possible Involvement of Enzymatic and Non-enzymatic Antioxidant System in Acquisition of Desiccation Tolerance of Maize Embryos [J]. Plant Diversity, 2009, 31(03): 253-259.
[6]	SONG Song-Quan , Patricia BERJAK , Norman W . PAMMENTER. Temporal Pattern of Changes in Desiccation Tolerance during Imbibition of Pisum sativum Seeds [J]. Plant Diversity, 2009, 31(03): 239-246.
[7]	DENG Zhi-Jun, XIANG Zhen-Yong^,, CHENG Hong-Yan, LI Yan-Jun, SONG Song-Quan^,. Preliminary Study on Development , Germination and Desiccation Tolerance of Jatropha curcas (Euphorbiaceae) [J]. Plant Diversity, 2008, 30(03): 355-359.
[8]	SHAO Yu-Tao; YIN Shou-Hua; LAN Qin-Ying; ZHANG Xiao-Yan. Developmental Changes in Relation to Desiccation Tolerance of Archontophoenix alexandrae (Palmae) Seeds [J]. Plant Diversity, 2006, 28(05): 515-522.
[9]	TANG An-Jun^,, LONG Chun-Lin, DAO Zhi-Ling ,SONG Song-Quan , TIAN Mei-Hua^,. Studies on Germination Characteristics and Storage Behavior of Trachycarpus fortunei Seeds [J]. Plant Diversity, 2005, 27(06): 657-662.
[10]	QIAN ChunMei,LEI YanBao,SONG SongQuan. Effect of Preimbibition on the DesiccationTolerance of Imbibed Vigna radiata Seeds [J]. Plant Diversity, 2003, 25(14): 1-3.
[11]	SONG Song-Quail, LONG Chun-Lin, YIN Shou-Hua , LAN Qin-Ying. Desiccation Behavior of Seeds and Their Molecular Mechanisms [J]. Plant Diversity, 2003, 25(04): 1-3.