Changes in Lysophospholipid and Degree of Unsaturated Membrane Lipids are Associated With Senescence in Arabidopsis Leaves

doi:10.7677/ynzwyj201312124

Abstract

Abstract:

Alterations of membrane fluidity and permeability are an inherent feature of deterioration associated with senescence in plants. With regard to membrane lipid constituents, the occurrence of lysophospholipid (lysoPL) is a signal of membrane damage, while changes in the double bond number of membrane lipids has important effects on membrane fluidity. In the present study, a lipidomics approach was used to study changes in lysoPL molecular species, and, in addition, the double bond index (DBI) of membrane lipids was calculated to indicate membrane fluidity in wild type (WS) and a phospholipid Dδ (PLDδ) knockout mutant of Arabidopsis during detachmentinduced, abscisic acid (ABA) or ethylenepromoted senescence. The results indicated that the content of total lysoPLs and some lysoPL molecular species increased markedly during ABApromoted senescence, while no significant change was detected during detachmentinduced and ethylenepromoted senescence. The DBI of total membrane lipids decreased during three senescence treatments; however, the pattern of change for each membrane lipid class differed between detachmentinduced and hormonepromoted senescence. Suppression of PLDδ attenuated lysoPLs accumulation during ABApromoted senescence, and slowed down the decrease of DBI of the total membrane lipids during ABA and ethylenepromoted senescence.

Key words: Arabidopsis, Phosphalipase Dδ, Ethylene, ABA, Senescence

CLC Number:

Q 945

JIA Yan-Xia-, LI Wei-Qi. Changes in Lysophospholipid and Degree of Unsaturated Membrane Lipids are Associated With Senescence in Arabidopsis Leaves[J]. Plant Diversity, 2013, 35(5): 569-577.

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