Plant Diversity ›› 2014, Vol. 36 ›› Issue (04): 485-496.doi: 10.7677/ynzwyj201413220

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Analysis of Endogenous Hormone Levels to Reveal the Retardation by Suppression of Phospholipase Dα1 in Arabidopsis Leaves during Hormonepromoted Senescence

 JIA  Yan-Xia, TAO  Fa-Qing, LI  Wei-Qi   

  1. Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany,
    Chinese Academy of Sciences, Kunming 650201, China
  • Received:2013-11-05 Online:2014-07-25 Published:2014-02-18
  • Supported by:

    The National Natural Science Foundation of China (NSFC 30670474, 30870571, and 31070262), Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China (O97C1211Z1), 100Talents Program of CAS (54O50617101)

Abstract:

Postharvest senescence is affected greatly by endogenous and exogenous hormones. The promotive effects of exogenous abscisic acid (ABA) and ethylene on senescence are retarded by suppression of phospholipase Dα1 (PLDα1) in detached Arabidopsis leaves. However, understanding about the roles of endogenous hormones in the retardation of postharvest senescence by the suppression of PLDα1 activity remains incomplete. Here we report changes in the levels  of endogenous ABA, methyl jasmonate (MeJA), indole3acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid (GA3) during leaf senescence induced by detachment and also accelerated by the application of exogenous ABA or ethylene in wildtype and PLDα1deficient Arabidopsis leaves. The responses of the five hormones to the three treatments showed the existence of two stages during the artificial senescence, and, for each hormone, the pattern during the early stage was identical. The retardation of senescence in PLDα1deficient plants was associated with changes in ABA, MeJA, ZR, and IAA levels, but was independent of changes in endogenous levels of GA3. The profiles of ZR and IAA suggest that changes in ZR and IAA levels might cause, and not simply be the consequence of, the retardation of senescence that is caused by suppression of PLDα1.

Key words: Leaf senescence, Postharvest physiology, Hormone, Phospholipase Dα1, Arabidopsis thaliana

CLC Number: 

  • Q 945