Plant Diversity ›› 2014, Vol. 36 ›› Issue (03): 279-284.doi: 10.7677/ynzwyj201413135

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Molecular Phylogeny of Ten Species of Oxytropis Based on psbAtrnH Sequences

 Lu  Ping, GAO  Li-Xia, JIN  Feng, EN  He-Ba-Ya-Er   

  1. College of Life Science and Technology of Inner Mongolia Normal University, Huhhot 010022, China
  • Received:2013-06-17 Online:2014-05-25 Published:2013-11-09

Abstract:

Total DNA from 56 individual plants collected in 28 sample sites of 10 Oxytropis species in Inner Mongolia was extracted in this research, and the psbAtrnH sequence was amplied and sequenced. Then the sequences were analyzed by ClustalX and MEGA 50. The phylogenetic trees were constructed by Maximum Likelihood methods in MEGA 50. The results showed as follows: (1) There were 50 variable sites, 36 informative site and 34% of the interspecific difference in the 10 Oxytropis species, and the GC contents of the psbAtrnH region were between 2318%~2572%. (2) The 10 species of Oxytropis were probably monophyletic, clearly separating from the Astragalus species and the bootstrap value was 99%. (3) The samples of Oxytropis glabra var. tenuis and Oglabra clustered a group independently, thus supported the opinion of treating Oxytropis glabra var. tenuis as a variant of Oglabra. (4)The samples of Overticillaris, Oracemosa and Oochrantha mixed. Therefore they showed a close relationship. We are congruent with the points in Flora of Intromongolica, the consensus putting the three species into Subgen. Euoxytropis Sect. Baicalia Stell. ex Bunge. (5) The samples of Ociliata and Oleptophylla clustered a group, which also supported the consensus which put them into sect. Xerobia Bunge. The investigation results indicated that psbAtrnH sequences provided the molecular evidences for the study of the phylogenetic relationships in the Oxytropis at subgenus, sects and species levels.

Key words: Oxytropis, Phylogenetic, psbA-trnH

CLC Number: 

  • Q 781