Plant Diversity ›› 2015, Vol. 37 ›› Issue (05): 605-610.doi: 10.7677/ynzwyj201514164

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The Determination of Nuclear DNA Content (2Cvalue) on Some Representative Genus and Species of Magnoliaceae

 YE  Lin-Jiang-1 , ZHANG  Zhi-Rong-3, SUN  Zhi-Xia-1, TIAN  Shuang-1、2   

  1. 1 Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China; 2 Department
    of Biology and Chemistry Engineering, Jingdezhen University, Jingdezhen, Jiangxi 333000, China;
    3 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany,
    Chinese Academy of Sciences, Kunming 650201, China
  • Received:2014-12-10 Online:2015-09-25 Published:2015-03-27
  • Supported by:

    国家自然科学基金 (41061006, 31160043) 和江西省高等学校科技落地计划 (KJLD14104)

Abstract:

The plant nuclear DNA content (2Cvalue) is a principal characteristic parmeter to describe biodiversity of species,which has important significance on the study of plant resources. In this study, we choosed 23 species from 10 representative genera (Magnolia、Michelia、Manglietia、Liriodendron、Talauma、 Paramichelia、Tsoongiodendron、Manglietiastrum、Kmeria、Parakmeria) of Magnoliaceae in China. All samples were determined using a flow cytometry technique with a standard of Zea mays (545pg/2C). The amount of nuclear DNA among these species ranged from 325pg (317850Mbp) to 1361pg (1331058Mbp) for Pgrandiflora, Mofficinalis subsp. biloba respectively, and the coefficient of variation (CV) were less than 5%. The results of the study will not only provide references for determination of the nuclear DNA content of Magnoliaceae and other plants, but also lay the foundation for the utilization and conservation of Magnoliaceae plant resources.

Key words: Magnoliaceae, Flow cytometry, Nuclear DNA content (2Cvalue), Genome size

CLC Number: 

  • Q 78