Plant Diversity ›› 2015, Vol. 37 ›› Issue (06): 861-870.DOI: 10.7677/ynzwyj201515032

• Articles • Previous Articles     Next Articles

Diversity of Culturable Bacteria Associated with Tuber panzhihuanensePinus armandii Ectomycorrhizosphere Soil

 MO  Shan-Ping-1、2、3 , ZHENG  Yi-1, SHANG  Li-1, LIU  Pei-Gui-2, WANG  Ran-2, XU  Fu-Qiang-2   

  1. 1 College of Resource and Environment, Yunnan Agricultural University, Kunming 650100, China; 2 Key Laboratory of Economic
    Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    3 College of Plant Protection, Yunnan Agricultural University, Kunming 650100, China
  • Received:2015-03-04 Online:2015-11-25 Published:2015-06-23
  • Supported by:

    National Natural Science Foundation of China (31460551, 31370070), the Joint Founds of the National Science Foundation of China and Yunnan Province Government (U1202262)


Truffles are edible hypogeous ectomycorrhizal fungi which have great economic importance for their organoleptic properties and have significant ecological interests for forestry. Although some new precious Chinese white truffle have been described constantly, the molecular mechanisms that control truffle body formation are largely unknown. It has been hypothesized that ectomycorrhizosphere soil communities may have influences on truffle production. Thus, isolation and molecular characterisation of culturable bacteria were carried out to investigate the bacteria diversity in mycorrhizosphere soil of Tuber panzhihuanensePinus armandii in this work. Sequencing results showed a significant presence mostly affiliated with Burkholderia was βProteobacteria (3098%). The second culturable fraction which dominated by Pseudomonas was γProteobacteria (288%) other isolates were mostly Phyllobacterium and Rhizobium, members of αProteobacteria (1467%), actinobacteria (125%) and Firmicutes (76%) represented by Arthrobacter and Bacillus, respectively. Chryseobacterium ureilyticum was the only bacterial strain belonging to Bacteroidetes. Similarities and differences of culturable bacterial community of ascocarps and ectomycorrhizosphere soil associated with Tuber were discussed.

Key words: Tuber panzhihuanense, Ectomycorrhizosphere soil, Bacteria diversity, 16S rDNA

CLC Number: