Germplasm resources of three wood plant species enriched with nervonic acid

doi:10.1016/j.pld.2022.01.004

Abstract

Abstract: Nervonic acid (NA) is a very-long-chain monounsaturated fatty acid with pharmaceutical and nutraceutical functions that plays an important role in treating several neurological disorders. One major source of NA is plant seed oil. Here we report fatty acid profiles of seeds and germplasm diversity of six plant species, including three woody plants with high amounts of NA-enriched seed oil, Malania oleifera, Macaranga adenantha, and M. indica. M. oleifera had the largest seed (average 7.40?g single seed), highest oil content (58.71%), and highest NA level (42.22%). The germplasm diversity of M. oleifera is associated with its habitat but not elevation. Seeds of M. adenantha contained higher NA levels (28.41%) than M.?indica (21.77%), but M.?indica contained a significantly higher oil content (29.22%) and seed yield. M.?adenantha germplasm varied among populations, with one population having seeds with high oil content (22.63%) and NA level (37.78%).Although M.?indica grow naturally at a range of elevations, no significant differences were detected between M.?indica populations. These results suggest that M. indica and M. oleifera have greater potential as a source of NA, which will contribute to constructing a germplasm resource nursery and establishing a selection and breeding program to improve the development of NA-enriched plants.

Key words: Nervonic acid, Germplasm resource, Malania oleifera, Macaranga adenantha, Macaranga indica, Diversity

Xing He, Tian-Quan Lu, Jiang-Ying Li, Ping Mao, Li Zhang, Guo-Wei Zheng, Bo Tian. Germplasm resources of three wood plant species enriched with nervonic acid[J]. Plant Diversity, 2022, 44(03): 308-315.

References

Amminger, G.P., Schafer, M.R., Klier, C.M., et al., 2012. Decreased nervonic acid levels in erythrocyte membranes predict psychosis in help-seeking ultra-high-risk individuals. Mol. Psychiatr. 17, 1150-1152
Blacklock, B.J., Jaworski, J.G., 2002. Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases. Eur. J. Biochem. 269, 4789-4798
Ghada, K., Mohamed, H., Sabrine, S., et al., 2018. A systematic comparison of 25 Tunisian plant species based on oil and phenolic contents, fatty acid composition and antioxidant activity. Ind. Crop. Prod. 123, 768-778
Guo, F.B., Wang, S.H., Wang, J., et al., 2018. Fruit yield and characters of wild Malania oleifera, a rare plant species in southwest China. Guihaia 38, 57-64
He, X., Li, D.Z.,Tian, B., 2021. Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid. Plant Divers. 43, 86-92
Huai, D., Zhang, Y., Zhang, C., et al., 2015. Combinatorial effects of fatty acid elongase enzymes on nervonic acid production in Camelina sativa. PLoS One 10, e0131755
Hui, Y.H., 1996. Bailey's Industrial Oil and Fat Products. vol. 1. Wiley-Interscience, New York, USA
Jart, A., 1978. The fatty acid composition of various cruciferous seeds. J. Am. Oil Chem. Soc. 55, 873-875
Jia, L., Zhou, J., 1987. The Oil Plants in China. Science Press, Beijing
Lai, J., 2006. Study on Conservation Biology of Rear and Precious Plant Malania oleifera. dissertation, Sichuan University
Lewkowicz, N., Pitek, P., Namiecinska, M., et al., 2019. Naturally occurring nervonic acid ester improves myelin synthesis by human oligodendrocytes. Cells 8, 786
Li, A.R., Mao, P., Li, Y.J., 2019a. Root hemiparasitism in Malania oleifera (olacaceae), a neglected aspect in research of the highly valued tree species. Plant Divers. 41, 347-351
Li, Q., Chen, J., Yu, X., et al., 2019b. A mini review of nervonic acid: source, production, and biological functions. Food Chem. 301, 125286
Litchfield, C., 1970. Tropaeolum speciosum seed fat: a rich source of cis-15-tetracosenoic andcis-17-hexacosenoic acids. Lipids 5, 144-146
Liu, F., Wang, P., Xiong, X., et al., 2021. A review of nervonic acid production in plants: prospects for the genetic engineering of high nervonic acid cultivars plants. Front. Plant Sci. 12, 626625
Lv, S., Wei, C., Huang, F., et al., 2016. Fruit and seed traits and adaptability to rocky desertification mountain of rare tree species Malania oleifera. Chinese J. Ecol. 35, 57-62
Ma, B.L., Liang, S.F., Zhao, D.Y., et al., 2004. Study on plants containing nervonic acid. Acta Bot. Boreali Occident. Sin. 24, 2362-2365
Mastebroek, H.D., Marvin, H.J.P., 2000. Breeding prospects of Lunaria annua L. Ind. Crop. Prod. 11, 139-143
Merrill, A.H., Schmelz, E.M., Wang, E., et al., 1997. Importance of sphingolipids and inhibitors of sphingolipid metabolism as components of animal diets. J. Nutr. 127(5 Suppl. l.), 830S-833S
Parke, D.V, Parke, A.L., 1999. Rape seed oil - an autoxidative food lipid. J. Clin. Biochem. Nutr. 26, 51-61
Qiu H, Michael G.G., 2008. Flora of China. Science Press, Beijing, (and Missouri Botanical Garden Press, St. Louis)
Qiu, H., 1996. Delectis Florae Reipublicae Popularis Sinicae Agendae Academiae Sinicae Edita, Flora Reipublicae Popularis Sinicae 44, Science Press, Beijing
Salas, J.J., Ohlrogge, J.B., 2002. Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases. Arch. Biochem. Biophys. 403, 25-34
Sargent, J.R., Coupland, K., Wilson, R., 1994. Nervonic acid and demyelinating disease. Med. Hypotheses 42, 237-242
Tang, T.F., Liu, X.M., Ling, M., et al., 2013. Constituents of the essential oil and fatty acid from Malania oleifera. Ind. Crop. Prod. 43, 1-5
Taylor, D.C., Francis, T., Guo, Y., et al., 2009. Molecular cloning and characterization of a KCS gene from Cardamine graeca and its heterologous expression in Brassica oilseeds to engineer high nervonic acid oils for potential medical and industrial use. Plant Biotechnol. J. 7, 925-938
Tian, B., Sun, M., Jayawardana, K., et al., 2020. Characterization of a PLDζ2 homology gene from developing castor bean endosperm. Lipids 55, 537-548
Vajreswari, A., Rao, P.S., Tulpule, P.G., 1991. Short-term effects of low erucic acid rapeseed oil and high erucic mustard oil on myocardial lipidosis of CFY strain of rats. J. Oil Technol.Assoc. India 23, 2-5
Vozella, V., Basit, A., Misto, A., et al., 2017. Age-dependent changes in nervonic acid-containing sphingolipids in mouse hippocampus. Biochim. Biophys. Acta 1862, 1502-1511
Whitmore, T.C., 2008. The Genus Macaranga, a Prodromus. Kew, Royal Botanic Gardens
Wu, Z., Raven, P., Hong, D., 2003. Flora of China. Vol. vol. 5 (Ulmaceae through Basellaceae). Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis
Xu, C.Q., Liu, H., Zhou, S.S., et al., 2019. Genome sequence of Malania oleifera, a tree with great value for nervonic acid production. GigaScience 8, 1-14
Yuan, Y., Xiao, H., Kang, H., et al., 2009. Fluorescence spectra study of a new toxic protein from Malania oleifera. Spectrosc. Spectr. Anal. 29, 777-780

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