High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation

doi:10.1016/j.pld.2016.07.002

Plant Diversity ›› 2016, Vol. 38 ›› Issue (05): 221-226.DOI: 10.1016/j.pld.2016.07.002

High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation

Jing Yang^a, Zerui Gao^b, Weibang Sun^a, Changqin Zhang^c

a. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China;
b. Yunnan Tobacco Industrial Hi-tech Material CO., LTD, Kunming, 650106, Yunnan, China;
c. Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China

收稿日期:2016-05-10 修回日期:2016-07-25 出版日期:2016-10-25 发布日期:2021-11-05
通讯作者: Changqin Zhang
基金资助:
This study was funded by the National Science Foundation of China (NSFCU1302262), and the Department of Forestry of Yunnan Province, project name “Rescue and conservation of PSESP species Craigia yunnanensis”. It was also supported by the Science and Technology Research Program of Kunming Institute of Botany, the Chinese Academy of Sciences (KIB2016005).

High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation

Jing Yang^a, Zerui Gao^b, Weibang Sun^a, Changqin Zhang^c

a. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China;
b. Yunnan Tobacco Industrial Hi-tech Material CO., LTD, Kunming, 650106, Yunnan, China;
c. Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China

Received:2016-05-10 Revised:2016-07-25 Online:2016-10-25 Published:2021-11-05
Contact: Changqin Zhang
Supported by:
This study was funded by the National Science Foundation of China (NSFCU1302262), and the Department of Forestry of Yunnan Province, project name “Rescue and conservation of PSESP species Craigia yunnanensis”. It was also supported by the Science and Technology Research Program of Kunming Institute of Botany, the Chinese Academy of Sciences (KIB2016005).

摘要/Abstract

摘要： Of the genus Craigia, widespread in the Tertiary, only two relict species survived to modern times. One species is now possibly extinct and the other one, Craigia yunnanensis, is severely endangered. Extensive surveys have located six C. yunnanensis populations in Yunnan province, southwest China. Using fluorescent amplified fragment length polymorphism (AFLP), the genetic diversity and population structure of these populations were examined. It was found that genetic diversity of C. yunnanensis was moderate at the species level, but low at regional and population levels. Analysis of population structure showed significant genetic differentiation between Wenshan and Dehong regions, apparently representing two geographically isolated for long time refuges. There are also clear indications of isolation between populations, which, together with anthropogenically caused decline of population size, will lead to general loss of the species genetic variation with subsequent loss of adaptive potential. To conserve the genetic integrity of C. yunnanensis, we recommend that ex-situ conservation should include representative samples from every population of the two differentiated regions (e.g. Wenshan and Dehong). The crosses between individuals originated from different regions should be avoided because of a high risk of outbreeding depression. As all the extant populations of C. yunnanensis are in unprotected areas with strong anthropogenic impact, there is no alternative to reintroduction of C. yunnanensis into suitable protected locations.

关键词: Craigia yunnanensis, Isolation, AFLP, Genetic diversity, Population genetic structure, Conservation genetics

Abstract: Of the genus Craigia, widespread in the Tertiary, only two relict species survived to modern times. One species is now possibly extinct and the other one, Craigia yunnanensis, is severely endangered. Extensive surveys have located six C. yunnanensis populations in Yunnan province, southwest China. Using fluorescent amplified fragment length polymorphism (AFLP), the genetic diversity and population structure of these populations were examined. It was found that genetic diversity of C. yunnanensis was moderate at the species level, but low at regional and population levels. Analysis of population structure showed significant genetic differentiation between Wenshan and Dehong regions, apparently representing two geographically isolated for long time refuges. There are also clear indications of isolation between populations, which, together with anthropogenically caused decline of population size, will lead to general loss of the species genetic variation with subsequent loss of adaptive potential. To conserve the genetic integrity of C. yunnanensis, we recommend that ex-situ conservation should include representative samples from every population of the two differentiated regions (e.g. Wenshan and Dehong). The crosses between individuals originated from different regions should be avoided because of a high risk of outbreeding depression. As all the extant populations of C. yunnanensis are in unprotected areas with strong anthropogenic impact, there is no alternative to reintroduction of C. yunnanensis into suitable protected locations.

Key words: Craigia yunnanensis, Isolation, AFLP, Genetic diversity, Population genetic structure, Conservation genetics

Jing Yang, Zerui Gao, Weibang Sun, Changqin Zhang. High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation[J]. Plant Diversity, 2016, 38(05): 221-226.

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High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation

High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation

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