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Plant Diversity ›› 2009, Vol. 31 ›› Issue (04): 317-325.DOI: 10.3724 SP.J.1143.2009.09036

• 研究论文 • 上一篇    下一篇

姜花属SRAP 分子标记连锁图谱构建

高丽霞1 , 刘 念2, 黄邦海3   

  1. 1 河池学院化学与生命科学系, 广西宜州 546300 ; 2 仲恺农业工程学院, 广东广州 510225; 3 广州市农业技术推广中心, 广东广州 510520

  • 收稿日期:2009-02-26 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 刘 念

Linkage Maps of the Genus Hedychium (Zingiberaceae) Based on SRAP

GAO Li-Xia1 , LIU Nian2, HUANG Bang-Hai3

  

  1. 1 Department of Chemistry and Life Science, Hechi University, Yizhou 546300 , China; 2 Zhongkai University of Agriculture and Engineering , Guangzhou 510225 , China ; 3 Guangzhou Municipal Center for the
    Promotion of Agricultural Technologies, Guangzhou 510520 , China
  • Received:2009-02-26 Online:2009-08-25 Published:2009-08-25

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摘要： 采用拟测交作图策略, 利用白姜花×圆瓣姜花的F1 群体87 个单株, 分别构建了父母本的基于SRAP标记的连锁图谱。通过筛选, 414 对引物中, 92 对引物可以检测到拟测交位点。在检测到的398 个拟测交位点中, 237 个来自于父本圆瓣姜花, 161 个来自于母本。经过卡方(χ2 ) 测验及连锁分析, 父本中, 203 个标记进入23 个连锁群, 覆盖了1 386. 8 cm; 母本中, 139 个标记进入18 个连锁群, 覆盖了917. 1 cm。

关键词: F1 群体, 连锁图谱, 拟测交作图

Abstract: In this study , using a pseudo-cross strategy , we constructed twomaps of Hedyhium , Hedychium coronarium and Hedychium forrestii based on SRAP markers . The mapping population consisted of 87 progenies, from a F1 population . A total of 414 primer pairs were screened and 92 pairs were considered . Among 398 loci , 237 loci were from the paternal parent and 161 were from the maternal parent . After χ2 test and linkage analysis, two maps were constructed as following: The paternal parent contained 203 loci and spanned 1 386 . 8 cm, which spread over 23 linkage groups . The maternal parent contained 139 loci and spanned 917 . 1 cm . These loci were distributed in 18 linkage groups .

Key words: F1 population

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