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Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 715-724.DOI: 10.7677/ynzwyj201313064

• 研究论文 • 上一篇    下一篇

中国秋海棠属 (秋海棠科) 植物的DNA条形码评价

 焦丽娟1、2, 税玉民1   

  1. 1 中国科学院昆明植物研究所生物多样性与生物地理学重点实验室,云南 昆明650201;
    2 中国科学院大学,北京100049
  • 收稿日期:2013-03-26 出版日期:2013-11-25 发布日期:2013-07-12
  • 基金资助:

    中国科学院大科学装置开放研究项目 (2009-LSF-GBOWS-01);中国国家自然科学基金 (NSFC40830209, 3170174);中国科学院独立研究项目 (KSCX2-EW-J-24)

Evaluating Candidate DNA Barcodes among Chinese Begonia (Begoniaceae) Species

 JIAO  Li-Juan-1、2, SHUI  Yu-Min-1   

  1. 1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-03-26 Online:2013-11-25 Published:2013-07-12



关键词: 秋海棠属, DNA条形码, ITS, matK, rbcL, trnH-psbA


Begonia is the biggest genus in the Begoniaceae and exhibits high morphological and ecological diversity, making intrageneric classification and species delimitation difficult, especially in the case of closely related species. In this respect, DNA barcoding has the advantage of achieving rapid species identification without relying on morphological characteristics, once a database containing speciesspecific sequences is established. In this study, we tested four candidate plant barcoding regions, including three chloroplast loci (rbcL, matK, trnHpsbA) and one nuclear locus (ITS), in 136 samples representing 26 species of Begonia. The results demonstrated that species identification based on rbcL, matK and trnHpsbA sequences was poor due to low interspecific variation. Conversely, ITS/ITS2 sequences showed significant intra and interspecific divergence enabling a high level of species discrimination (96%-100%). Consequently, ITS/ITS2 could be considered as a potential barcode for identifying Begonia species, which supports the proposal by the China Plant BOL Group that ITS/ITS2 should be incorporated into the core barcode for seed plants.

Key words: Begonia, DNA barcoding, ITS, matK, rbcL, trnH-psbA