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Plant Diversity ›› 2010, Vol. 32 ›› Issue (02): 141-146.DOI: 10.3724/SP.J.1143.2010.09239

• 研究论文 • 上一篇    下一篇

拟南芥SDIR1基因转录的选择性剪接

武晓璐,汤晓倩,于丽霞,姚有林,鄢波   

  1. 西南林学院园林学院, 云南 昆明 650224
  • 收稿日期:2009-11-30 出版日期:2010-04-25 发布日期:2010-06-29
  • 通讯作者: 鄢波

Alternative Splicing of SDIR1 Gene in Arabidopsis thaliana (Cruciferae)

WU XiaoLu, TANG XiaoQian, YU LiXia, YAO YouLin, YAN Bo   

  1. Faculty of Landscape Architecture; Southwest Forestry College, Kunming 650224, China
  • Received:2009-11-30 Online:2010-04-25 Published:2010-06-29
  • Contact: YAN Bo

摘要: 采用PCR及RTPCR法分别克隆了拟南芥SDIR1基因的DNA和cDNA序列。根据序列比对分析结果,发现了3种不同的转录本,提示SDIR1基因的转录中存在选择性剪接。3种转录本的长度分别为822 bp、691 bp和666 bp,依次命名为:SDIR1822、SDIR1691、SDIR1666。与SDIR1基因的DNA序列及已报道的SDIR1 cDNA序列比较,除转录本SDIR1822包含了完整的编码序列外,其余2种转录本的编码序列都存在不同长度的缺失。其中,SDIR1691缺失了131 bp的片段:第2外显子3′端缺失33 bp,第3外显子53 bp全部缺失,第4外显子5′端缺失45 bp;转录本SDIR1666缺失了156 bp的片段:第3外显子3′端缺失18 bp,第4外显子5′端缺失138 bp。进而随机挑取101个克隆子对三种转录本的表达比例进行初步分析,结果表明3种分子的比值为SDIR1822∶SDIR1691∶SDIR1666 = 2600∶133∶100,反映出SDIR1基因不同转录本在拟南芥中的相对表达量。

关键词: SDIR1基因;选择性剪接;拟南芥

Abstract: Sequences of DNA and cDNA of SDIR1 gene in Arabidopsis thaliana were isolated by using PCR and RTPCR. The alignment result showed that there were three different transcripts in Arabidopsis thaliana, which indicated that there were alternative splicing pneumonia in the transcription of SDIR1 gene. The length of three transcripts were 822 bp, 691 bp and 666 bp, respectively, and were named as SDIR1822, SDIR1691 and SDIR1666 subsequently. Compared with the reported sequences of DNA and cDNA in Arabidopsis thaliana, SDIR1822 contained intact coding domain, while the others missed some nucleotides in coding domain. SDIR1691 missed 131 bp which contained 33 bp in the 3′ end of the second exon, 53 bp of all the third exon and 45 bp in the 5′ end of the fourth exon. SDIR1666 missed 156 bp which contained 18 bp in the 3′ end of the third exon and 138 bp in the 5′ end of the fourth exon. The expression ratio of three different transcripts SDIR1822, SDIR1691 and SDIR1666 was 2600∶133∶100 analyzed by selecting 101 clones, which might indicate the relative content of different transcripts of SDIR1 gene in Arabidopsis thaliana.

Key words: SDIR1 gene

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