不同地理种源云南红豆杉的遗传多样性与紫杉醇含量相关性分析

Plant Diversity ›› 2009, Vol. 31 ›› Issue (06): 493-498.

不同地理种源云南红豆杉的遗传多样性与紫杉醇含量相关性分析

于晓芹^{1 , 2} , 刘锡葵¹ , 顾志建¹

1 中国科学院昆明植物研究所, 云南昆明 650204; 2 中国科学院研究生院, 北京 100049

收稿日期:2009-02-10 出版日期:2009-12-25 发布日期:2009-12-25
通讯作者: 顾志建

Analysis of Relationship Between Genetic Diversity and Taxol Content of Taxus wallichiana var. wallichiana in Different Provenance

YU Xiao-Qin^{1 ,2} , LIU Xi-Kui¹ , GU Zhi-Jian¹

1 Kunming Institute of Botany , Chinese Academy of Sciences , Kunming 650204 , China ;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China

Received:2009-02-10 Online:2009-12-25 Published:2009-12-25
Contact: GU Zhi-Jian

摘要/Abstract

摘要： 应用AFLP 和HPLC 方法研究了云南红豆杉( Taxus wallichiana var. wallichiana ) 的遗传多样性与紫杉醇含量之间的关系。AFLP 指纹图谱显示7 个居群谱带明显分为两种式样。PopGene1. 31 和Arlequin3 . 1 软件分析结果表明: 云南红豆杉居群内遗传比较稳定, 居群间遗传分化极其显著( F_st = 0 .67 )。测定当年生小枝叶紫杉醇含量结果表明: 紫杉醇含量在居群间和居群内个体差异都较大。天然林中潞西居群的紫杉醇平均含量为0 . 0185% ; 腾冲和永德居群的紫杉醇含量比较低, 分别为0 . 0049%和0 . 0087% ; 禄丰栽培居群紫杉醇含量比较高, 平均为0 . 0225%。在居群水平上, 遗传多样性和紫杉醇含量有一定程度的关联, 但在个体水平上明显无对应关系。

关键词: 云南红豆杉, 遗传多样性, 紫杉醇含量, AFLP, HPLC

Abstract: We studied the relationship between genetic diversity and taxol content of Taxus wallichiana var. wallichiana using AFLP and HPLC . The results of AFLP fingerprinting indicated that two patterns were discovered among seven populations. Through PopGene 1 . 31 and Arlequin 3. 1 software , stable genetics within population and significant differentiation among populations (F_st= 0. 67 ) were revealed . However, taxol content was markedly different within and among populations when testing the one-year twigs . Among wild forests, the highest taxol content was found in Luxi population which average percentage was 0. 0185%. While the taxol content level was low in Tengchong and Yongde populations , which were 0. 0049% and 0. 0087% respectively . The cultivated population in Lufeng showed the highest taxol content level which was 0. 0225% comparing with that in the populations of wild forests. There were relations between genetic diversity and taxol
content at the population level to some extent , while no corresponding relations were found at the individual level .

Key words: Taxus wallichiana var. wallichian

中图分类号:

Q 16

于晓芹1 , 2 , 刘锡葵1 , 顾志建1 . 不同地理种源云南红豆杉的遗传多样性与紫杉醇含量相关性分析[J]. Plant Diversity, 2009, 31(06): 493-498.

YU Xiao-Qin^, , LIU Xi-Kui , GU Zhi-Jian. Analysis of Relationship Between Genetic Diversity and Taxol Content of Taxus wallichiana var. wallichiana in Different Provenance[J]. Plant Diversity, 2009, 31(06): 493-498.

[1]	Jing Yang, Zerui Gao, Weibang Sun, Changqin Zhang. High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation[J]. Plant Diversity, 2016, 38(05): 221-226.
[2]	呼慧丽, 李波, 胡菀, 孙志霞, 张志勇, 范邓妹. 南天竹微卫星标记开发及特性分析[J]. Plant Diversity, 2015, 37(2): 141-144.
[3]	余亚莹1, 邵高能1, 圣忠华1, 蒋汉伟2, 贺记外1, 孙园园2, 蔡怡聪1, 胡培松1**, 唐绍清1. 国内外香稻资源遗传多样性研究[J]. Plant Diversity, 2015, 37(06): 871-880.
[4]	徐玉仙, 张微微, 莫海波, 李春, 曹建国, 田代科. 基于ESTSSR标记的莲属种质资源遗传多样性分析[J]. Plant Diversity, 2015, 37(05): 595-604.
[5]	李苗苗, MEEGAHAKUMBURA M. Kasun, 严丽君, 刘杰, 高连明. 核基因组微卫星标记揭示大理茶参与了普洱茶的驯化过程[J]. Plant Diversity, 2015, 37(01): 29-37.
[6]	叶林江, 王静, 孙鹏, 董书鹏, 张志勇. 四种栲属植物微卫星标记在钩栲中的通用性检测[J]. Plant Diversity, 2014, 36(04): 443-448.
[7]	黄家林, 李树云, 胡虹. 珍稀濒危植物硬叶兜兰的遗传多样性及遗传结构研究[J]. Plant Diversity, 2014, 36(02): 209-218.
[8]	官萌萌1、2, 马瑞1, 龚洵1. 金沙江河谷特有植物罂粟莲花的保护遗传学[J]. Plant Diversity, 2013, 35(5): 555-562.
[9]	赵凯1、2, 王德元2, 张文娟2, 邵剑文2 . 都支杜鹃遗传多样性的ISSR分析[J]. Plant Diversity, 2013, 35(5): 578-584.
[10]	何庆元1、2, 李正鹏1, 吴萍1, 杨红燕2, 王松华1. 起始密码子区域相关序列多态性的开发及在苜蓿遗传多样性上的应用[J]. Plant Diversity, 2013, 35(1): 48-54.
[11]	杨春1、2, 刘爱忠1. 小桐子EST-SSR分子标记的开发与种质遗传多样性分析[J]. Plant Diversity, 2011, 33(5): 529-534.
[12]	蒋志敏, 陈玉霞, 包颖. 黄河三角洲柽柳居群的遗传结构和遗传分化[J]. Plant Diversity, 2011, 33(4): 403-408.
[13]	陈香波1, 田旗2. 夏蜡梅遗传多样性迁地保护取样策略研究[J]. Plant Diversity, 2010, 32(S17): 74-80.
[14]	刘阳1、2, 杨世雄3, 高立志1 . 云南古茶园栽培大叶茶和大理茶群体的叶绿体RPL32-TRNL核苷酸变异和遗传分化[J]. Plant Diversity, 2010, 32(05): 427-434.
[15]	袁菊红1，胡绵好2，夏冰3 . 利用SRAP标记分析中国野生石蒜的遗传多样性[J]. Plant Diversity, 2010, 32(03): 255-262.