四种栲属植物微卫星标记在钩栲中的通用性检测

doi:10.7677/ynzwyj201413191

摘要/Abstract

摘要：

钩栲（Castanopsis tibetana）是中国亚热带常绿阔叶林具有代表性的建群种之一，由于生境片段化的影响，其遗传多样性和进化潜力受到严重威胁。为研究钩栲遗传多样性及群体遗传特征，本研究从来自中华栲（Castanopsis chinensis）、苦槠栲（Csclerophylla）、尖叶栲（Ccuspidata）、日本栲（Csieboldii）4种栲属植物的51个微卫星位点中，筛选出35个在钩栲中具有稳定PCR扩增的位点；采用4个钩栲群体24个个体进行检测，发现11个位点具有较低的多态性，其中等位基因数目（NA）为2~7，平均45；观测杂合度（HO）为0083~0917，平均0505；预期杂合度（HE）为0236~0667，平均0471。研究结果对钩栲遗传多样性研究和保护具有重要作用。

关键词: 钩栲, 微卫星, 通用性, 遗传多样性

Abstract:

Castanopsis tibetana is a typical and dominant component in subtropical evergreen broadleaved forests of China. However, its genetic diversity and longterm persistence have been jeopardized by habitat fragmentation. To examine its genetic diversity and genetic structure, crossspecies amplification of 51 microsatellite loci which are derived from four congeneric species, ie., Castanopsis chinensis, Csclerophylla, Ccuspidata and Csieboldii, were tested in Ctibetana.Thirtyfive loci were successfully amplified in Ctibetana, among them 11 were polymorphic across four wild populations of Ctibetana (24 individuals in total). Genetic diversity of four Ctibetana populations (Jiangxi) was relatively low. The number of alleles (NA) ranged from 2 to 7 (average 45) and the ranges of observed (HO) and expected (HE) heterozygosities were 0505 and 0471，respectively. The results will facilitate the study and conservation of genetic diversity of Ctibetana.

Key words: Castanopsis tibetana, Microsatellite, Transferability, Genetic diversity

中图分类号:

Q 16

叶林江, 王静, 孙鹏, 董书鹏, 张志勇. 四种栲属植物微卫星标记在钩栲中的通用性检测[J]. Plant Diversity, 2014, 36(04): 443-448.

YE Lin-Jiang, WANG Jing, SUN Peng, DONG Shu-Peng, ZHANG Zhi-Yong. The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana (Fagaceae)[J]. Plant Diversity, 2014, 36(04): 443-448.

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