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Plant Diversity ›› 2014, Vol. 36 ›› Issue (04): 443-448.DOI: 10.7677/ynzwyj201413191

• 研究论文 • 上一篇    下一篇


 叶林江, 王静, 孙鹏, 董书鹏, 张志勇   

  1. 江西农业大学亚热带生物多样性实验室,江西 南昌330045
  • 收稿日期:2013-09-19 出版日期:2014-07-25 发布日期:2014-03-25
  • 基金资助:

    国家自然科学基金 (31160043, 31160082);国家科技支撑计划 (2012BAC11B02)

The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana (Fagaceae)

 YE  Lin-Jiang, WANG  Jing, SUN  Peng, DONG  Shu-Peng, ZHANG  Zhi-Yong   

  1. Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2013-09-19 Online:2014-07-25 Published:2014-03-25
  • Supported by:

    国家自然科学基金 (31160043, 31160082);国家科技支撑计划 (2012BAC11B02)


钩栲(Castanopsis tibetana)是中国亚热带常绿阔叶林具有代表性的建群种之一,由于生境片段化的影响,其遗传多样性和进化潜力受到严重威胁。为研究钩栲遗传多样性及群体遗传特征,本研究从来自中华栲(Castanopsis chinensis)、苦槠栲(Csclerophylla)、尖叶栲(Ccuspidata)、日本栲(Csieboldii)4种栲属植物的51个微卫星位点中,筛选出35个在钩栲中具有稳定PCR扩增的位点;采用4个钩栲群体24个个体进行检测,发现11个位点具有较低的多态性,其中等位基因数目(NA)为2~7,平均45;观测杂合度(HO)为0083~0917,平均0505;预期杂合度(HE)为0236~0667,平均0471。研究结果对钩栲遗传多样性研究和保护具有重要作用。

关键词: 钩栲, 微卫星, 通用性, 遗传多样性


Castanopsis tibetana is a typical and dominant component in subtropical evergreen broadleaved forests of China. However, its genetic diversity and longterm persistence have been jeopardized by habitat fragmentation. To examine its genetic diversity and genetic structure, crossspecies amplification of 51 microsatellite loci which are derived from four congeneric species, ie., Castanopsis chinensis, Csclerophylla, Ccuspidata and Csieboldii, were tested in Ctibetana.Thirtyfive loci were successfully amplified in Ctibetana, among them 11 were polymorphic across four wild populations of Ctibetana (24 individuals in total). Genetic diversity of four Ctibetana populations (Jiangxi)  was relatively low. The number of alleles (NA) ranged from 2 to 7 (average 45) and the ranges of observed (HO) and expected (HE) heterozygosities were 0505 and 0471,respectively. The results will facilitate the study and conservation of genetic diversity of Ctibetana.

Key words: Castanopsis tibetana, Microsatellite, Transferability, Genetic diversity