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Plant Diversity ›› 2014, Vol. 36 ›› Issue (05): 595-602.DOI: 10.7677/ynzwyj201413106

• 研究论文 • 上一篇    下一篇

植物在缺钾胁迫中膜稳定性的变化

王丹丹1、2, 郑国伟1, 李唯奇1、3   

  1. 1 中国科学院昆明植物研究所中国西南野生生物种质资源库,云南 昆明650201; 2 中国科学院大学,北京100039;3 红河学院生物系,云南 蒙自661100
  • 收稿日期:2013-05-05 出版日期:2014-09-25 发布日期:2013-07-15
  • 基金资助:

    The National Natural Science Foundation of China (NSFC 30670474, 30870571 and 31070262)

Changes of Membrane Stability in PotassiumStressed Plants

WANG Dan-Dan-1、2, ZHENG Guo-Wei-1, LI Wei-Qi-1、3   

  1. 1 The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100039, China; 3 Biology Department, Honghe University, Mengzi 661100, China
  • Received:2013-05-05 Online:2014-09-25 Published:2013-07-15
  • Supported by:

    The National Natural Science Foundation of China (NSFC 30670474, 30870571 and 31070262)

摘要:

植物维持膜的功能是其抵御胁迫的关键问题,而维持膜功能必须要保持膜的稳定性和合适的流动性。我们前期的研究发现植物主要是通过积累叶片膜脂和保持根部膜脂基本不变来适应长期缺钾。在本研究中,以拟南芥和其具有耐受缺钾胁迫特性的近缘种须弥芥为对象,研究了与膜的流动性密切相关的双键指数(double bond index, DBI)的变化,发现长期缺钾条件下,两种植物叶片中总的DBI保持不变,根部总的DBI略有降低。同时研究了与膜稳定性密切相关的溶血磷脂的含量和DGDG/MGDG以及PC/PE这两个比值的变化,发现长期缺钾后拟南芥和须弥芥叶片中溶血磷脂的总量呈上升趋势,根部溶血磷脂总量基本保持不变;无论在对照还是缺钾条件下,拟南芥溶血磷脂的总含量要高于须弥芥。须弥芥叶片具有更高的DGDG/MGDG值,根部具有更高的PC/PE值,说明长期缺钾条件下须弥芥膜的稳定性可能更好。这可能是须弥芥耐缺钾的原因之一。

关键词: 须弥芥, 拟南芥, 耐缺钾, 膜流动性, 膜稳定性

Abstract:

The maintenance of membrane function is critical to the ability of plants to resist environmental stresses; specifically, the stability and appropriate fluidity of membranes are crucial to their normal function. We previously demonstrated that plants adapt to longterm potassium (K+) deficiency by accumulation of membrane lipids in leaves and maintenance of the lipid composition in roots. In this study, which involved Arabidopsis thaliana and its K+deficiencytolerant relative Crucihimalaya himalaica, we first calculated the doublebond index (DBI) as an indicator of membrane fluidity. After exposure to longterm K+deficiency stress, the DBI of the total lipids in leaves of Athaliana and Chimalaica showed no significant changes, whereas the DBI of the total lipids in the roots of these species showed slight increases. Changes in lysophospholipids (lysoPLs) levels, and digalactosyldiacylglycerol/monogalactosyldiacylglycerol (DGDG/MGDG) and phosphatidylcholine/phosphatidylethanolamine (PC/PE) ratios, all of which strongly reflect membrane stability, were also studied in K+stressed Athaliana and Chimalaica. After longterm K+ deficiency, total lysoPLs levels increased in Athaliana and Chimalaica leaves, but showed no significant changes in roots. DGDG/MGDG and PC/PE ratios were higher in Chimalaica leaves and roots than in those of Athaliana. These results indicate that Chimalaica exhibits superior membrane stability compared with Athaliana. This may explain its superior growth and tolerance under K+deficient conditions.

Key words: Crucihimalaya himalaica, Arabidopsis thaliana, Tolerance of K+ deficiency, Membrane fluidity, Membrane stability

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