Plant Diversity ›› 2018, Vol. 40 ›› Issue (01): 41-44.DOI: 10.1016/j.pld.2017.09.003

• Articles • Previous Articles    

Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing

Masaya Yamamotoa, Yoshihiro Handa,b, Hiroki Aiharab, Hiroaki Setoguchia   

  1. a Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo-ku, Kyoto 606-8501, Japan;
    b FASMAC Co., Ltd., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan
  • Received:2017-03-23 Revised:2017-09-06 Published:2021-11-05
  • Contact: Masaya Yamamoto
  • Supported by:
    This research was financially and technically supported by FASMAC Co., Ltd. (Kanagawa, Japan) and The Environment Research and Technology Development Fund (#4-1403).

Abstract: Primula reinii (Primulaceae), a perennial herb belonging to the Primula section Reinii, occurs on wet, shaded rocky cliffs in the mountains of Japan. This threatened species comprises four varieties; these plants are very localized and rare in the wild. In this study, 43 microsatellite markers were developed using MiSeq sequencing to facilitate conservation genetics of these critically endangered primroses. We developed novel microsatellite markers for three varieties of P. reinii, and tested its polymorphism and genetic diversity using natural populations. These novel markers displayed relatively high polymorphism; the number of alleles and expected heterozygosities ranged from 2 to 6 (mean=3.2) and 0.13 to 0.82 (mean=0.45), respectively. All loci were in HardyeWeinberg equilibrium. These microsatellite markers will be powerful tools to assess P. reinii genetic diversity and develop effective conservation and management strategies.

Key words: Microsatellites, Polymorphism, MiSeq, Critically endangered plant, Primula reinii