Plant Diversity ›› 2015, Vol. 37 ›› Issue (4): 416-422.doi: 10.7677/ynzwyj201514144

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The Transferability of SSR Markers in Seven Asteraceae Species to Taxa in the Natural Hybrid Zone between Ligularia duciformis and Lparadoxa

 ZHANG  Rong-1、2, FENG  Xiu-Yan-1、2, GONG  Xun-1   

  1. 1 Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201,China; 2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-11-04 Online:2015-07-25 Published:2014-12-23
  • Supported by:

    国家自然科学基金 (31470336)

Abstract:

Ligularia × maoniushanensis (Asteraceae) is a natural hybrid species of Lduciformis and Lparadoxa. In order to study hybridization and genetic structure of taxa in the hybrid zones between Lduciformis and Lparadoxa, crossspecies SSR primers were screened using 94 individuals of two hybrid zones’ hybrids and its parents from 145 nuclear SSR primers which had been developed from Ligularia, Farfugium, Senecio and Helianthus. The results indicated that 54 loci were successfully amplified at least one taxon of three taxa. A total of 17 loci were successfully amplified at three taxa, of which 11 were polymorphic in L. × maoniushanensis, and the number of alleles (NA) ranged from 2 to 14 (average 48) and the ranges of observed (HO) and expected (HE) heterozygosities were 0053~1000 and 0051~0887, respectively. Of which 9 were polymorphic in Lduciformis, and the number of alleles (NA) ranged from 3 to 12 (average 74) and the ranges of observed (HO) and expected (HE) heterozygosities were 0200~0971 and 0283~0844, respectively. Of which 10 were polymorphic in Lparadoxa, the number of alleles (NA) ranged from 4 to 15 (average 7.6) and the ranges of observed (HO) and expected (HE) heterozygosities were 0025~0974 and 0097~0846, respectively. The results will facilitate the further analysis of genetic structure and hybrid extent about taxa in hybrid zone between Lduciformis and Lparadoxa.

Key words: L. ×, maoniushanensis, L. duciformis, L. paradoxa, SSR, Polymorphism, Transferability

CLC Number: 

  • Q 75