The Transferability of SSR Markers in Seven Asteraceae Species to Taxa in the Natural Hybrid Zone between Ligularia duciformis and Lparadoxa

doi:10.7677/ynzwyj201514144

Abstract

Abstract:

Ligularia × maoniushanensis (Asteraceae) is a natural hybrid species of Lduciformis and Lparadoxa. In order to study hybridization and genetic structure of taxa in the hybrid zones between Lduciformis and Lparadoxa, crossspecies SSR primers were screened using 94 individuals of two hybrid zones’ hybrids and its parents from 145 nuclear SSR primers which had been developed from Ligularia, Farfugium, Senecio and Helianthus. The results indicated that 54 loci were successfully amplified at least one taxon of three taxa. A total of 17 loci were successfully amplified at three taxa, of which 11 were polymorphic in L. × maoniushanensis, and the number of alleles (NA) ranged from 2 to 14 (average 48) and the ranges of observed (HO) and expected (HE) heterozygosities were 0053~1000 and 0051~0887, respectively. Of which 9 were polymorphic in Lduciformis, and the number of alleles (NA) ranged from 3 to 12 (average 74) and the ranges of observed (HO) and expected (HE) heterozygosities were 0200~0971 and 0283~0844, respectively. Of which 10 were polymorphic in Lparadoxa, the number of alleles (NA) ranged from 4 to 15 (average 7.6) and the ranges of observed (HO) and expected (HE) heterozygosities were 0025~0974 and 0097~0846, respectively. The results will facilitate the further analysis of genetic structure and hybrid extent about taxa in hybrid zone between Lduciformis and Lparadoxa.

Key words: L. ×, maoniushanensis, L. duciformis, L. paradoxa, SSR, Polymorphism, Transferability

CLC Number:

Q 75

ZHANG Rong-, FENG Xiu-Yan-, GONG Xun. The Transferability of SSR Markers in Seven Asteraceae Species to Taxa in the Natural Hybrid Zone between Ligularia duciformis and Lparadoxa[J]. Plant Diversity, 2015, 37(4): 416-422.

References

邹喻苹, 葛颂, 王晓东, 2001. 系统与进化植物学中的分子标记［M］. 北京: 科学出版社, 68—80
刘尚武, 1989. 菊科-千里光族［A］// 林镕, 刘尚武, 中国植物志第77卷第2分册［M］. 北京: 科学出版社, 4—115
Ahrens CW, James EA, 2013. Characterization of microsatellite markers for the vulnerable grassland forb Senecio macrocarpus (Asteraceae)［J］. Applications in Plant Sciences, 1 (11): 1300041
Barbara T, PalmaSilva C, Paggi GM et al., 2007. Crossspecies transfer of nuclear microsatellite markers: potential and limitations［J］. Molecular Ecology, 16 (18): 3759—3767
Brennan AC, Barker D, Hiscock SJ et al., 2012. Molecular genetics and quantitative trait divergence associated with recent homoploid hybrid speciation: a study of Senecio squalidus (Asteraceae) ［J］. Heredity, 108 (2): 87—95
Doyle J, 1991. DNA protocols for plants CTAB total DNA isolation［A］// Hewitt GM, Johnston A eds., Molecular Techniques in Taxonomy［M］. Berlin: Springer, 283—293
He P (何平), 1998. Abundance, polymorphism and applications of microsatellite in eukaryote［J］. Hereditas (遗传), 20 (4): 42—47
Heesacker A, Kishore VK, Gao WX et al., 2008. SSRs and INDELs mined from the sunower EST database: abundance, polymorphisms, and crosstaxa utility［J］. Theoretical and Applied Genetics, 117 (7): 1021—1029
Kalia RK, Rai MK, Kalia S et al., 2011. Microsatellite markers: an overview of the recent progress in plants［J］. Euphytica, 177 (3): 309—334
Lade JA, Murray ND, Marks CA et al., 1996. Microsatellite differentiation between Philip Island and mainland Australian populations of the red fox Vulpes vulpes［J］. Molecular Ecology, 5 (1): 81—87
Liu GQ, Hegarty MJ, Edwards KJ et al., 2004. Isolation and characterization of microsatellite loci in Senecio［J］. Molecular Ecology Notes, 4 (4): 611—614
Liu JQ, Liu SW, Ho TN et al., 2001. Karyological studies on the SinoHimalayan genus, Cremanthodium (Asteraceae: Senecioneae)［J］. Botanical Journal of the Linnean Society, 135 (2): 107—112
Liu JQ, Wang YJ, Abbott RJ et al., 2006. Radiation and diversification within the LigulariaCremanthodiumParasenecio complex (Asteraceae) triggered by uplift of the QinghaiTibetan Plateau［J］. Molecular Phylogenetics and Evolution, 38 (1): 31—49
Liu SW (刘尚武), Deng DS (邓德山), Liu JQ (刘建全), 1994. The origin, evolution and distribution of Ligularia Cass. (Compositae) ［J］. Acta Phytotaxonomica Sinica (植物分类学报), 32 (6): 514—524
Liu SW, Illarionova ID, 2011. Ligularia［A］// Wu ZY, Raven PH, Hong DY eds., Flora of China［M］. Beijing: Science Press & St. Louis: Missouri Botanical Garden Press, 371—544
Mao CL, Pan YZ, Wang JF et al., 2009. Isolation and characterization of microsatellite markers for Ligularia hodgsonii Hook. (Asteraceae) ［J］. Conservation Genetics, 10 (6): 1853—1855
Nomura N, Fujiwara K, Takaso T et al., 2009. Development and characterisation of microsatellite loci in Farfugium japonicum (Asteraceae) ［J］. Conservation Genetics, 10 (6): 1093—1095
Nybom H, 2004. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plant［J］. Molecular Ecology, 13 (5): 1143—1155
Pan YZ, Shi SH, Gong X et al., 2008. A Natural Hybrid Between Ligularia paradoxa and Lduciformis (Asteraceae, Senecioneae) From Yunnan, China［J］. Annals of the Missouri Botanical Garden, 95 (3): 487—494
Peakall R, Smouse PE, 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research［J］. Molecular Ecology Notes, 6 (1): 288—295
Pelser PB, Nordenstam B, Kadereit JW et al., 2007. An ITS phylogeny of tribe Senecioneae (Asteraceae) and a new delimitation of Senecio L.［J］. Taxon, 56 (4): 1077—1104
Pelser PB, Kennedy AH, Tepe EJ et al., 2010. Patterns and causes of incongruence between plastid and nuclear Senecioneae (Asteraceae) phylogenies［J］. American Journal of Botany, 97 (5): 856—873
Roux JJ, Wieczorek AM, 2007. Isolation and characterization of polymorphic microsatellite markers from fireweed, Senecio madagascariensis Poir. (Asteraceae) ［J］. Molecular Ecology Notes, 7 (2): 327—329
Saito Y, Hattori M, Iwamoto Y et al., 2011. Overlapping chemical and genetic diversity in Ligularia lamarum and Ligularia subspicata. Isolation of ten new eremophilanes and a new secobakkane compound［J］. Tetrahedron, 67 (12): 2220—2231
Shimizu A, Watanabe S, Hanai R et al., 2014. Chemical and genetic studies on hybrid of Ligularia subspicata and Ligularia cyathiceps collected in Yunnan Province of China［J］. Chemistry & Biodiversity, 11 (9): 1438—1448
Wang L (王丽), Zhao GF (赵桂仿), 2005. Microsatellite primers shared by different plant species and genera［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 25 (8): 1540—1546
Wu K, Tanksley SD, 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice［J］. Molecular and General Genetics MGG, 24 (12): 225—235
Yatabe Y, Kane NC, ScottiSaintagne C et al., 2007. Rampant gene exchange across a strong reproductive barrier between the annual sunflowers, Helianthus annuus and Hpetiolaris［J］. Genetics, 175 (4): 1883—1893
Yeh FC, Yang RC, Boyle TBJ et al., 1997. POPGENE, the user friendly shareware for population genetic analysis［R］. Molecular Biology and Biotechnology Centre, University of Alberta, Canada, 10
Ye LJ (叶林江), Wang J (王静), Sun P (孙鹏) et al., 2014. The transferability of nuclear molecular markers in four Castanopsis species to Castanopsis tibetana (Fragaceae) ［J］. Plant Diversity and Resources (植物分类与资源学报), 36 (4): 443—448
Yu JJ, Kuroda C, Gong X, 2011. Natural hybridization and introgression in sympatric Ligularia species (Asteraceae, Senecioneae) ［J］. Journal of Systematics and Evolution, 49 (5): 438—448
Yu JJ, Pan L, Pan YZ et al., 2014. Natural hybrids between Ligularia vellerea and Lsubspicata (Asteraceae: Senecioneae) ［J］. Plant Diversity and Resources (植物分类与资源学报), 36 (02): 219—226
Zane L, Barglloni L, Patarnello T, 2002. Strategies for microsatellite isolation: a review［J］. Molecular Ecology, 11 (1): 1—16

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