Plant Diversity ›› 2010, Vol. 32 ›› Issue (02): 151-157.doi: 10.3724/SP.J.1143.2010.09190
• Articles •
JI HongChun1,2, SU ZhiXian1,2, YANG Jun1, BIAN ChunXiang1, HU JinYao2, QI Gang 2
A coldinduced plasma membrane protein which plays a protective role in avoiding freezing injury for some plants is one of hydrophobic proteins. A gene, named as DiRCI, was isolated from the cDNA library of Davidia involucrata, containing an open reading frame of 174 bp flanked by a 92 bp 5′untranslated sequence and a long 273 bp 3′noncoding region. Alignment analysis indicated that the deduced amino acid sequence of 57 amino acids was high conserved with the MpRCI from Plantago asiatica, and the identity was 895%. Semiquantitative reverse transcriptionpolymerase chain reaction (RTPCR) analysis showed that DiRCI wasn′t detected in various organs of Dinvolucrata above 25℃. When the plant grew under 8℃, the gene expressed in leaves, leafstalk and seeds, but not the roots. The expression level gradually increased after 4 h of the cold treatment and reached a maximum during 24-48 h, and then decreased. The results indicated the gene did have a relation with cold stress in plant and was predicted to be one DiRCI gene. The present data not only enrich gene resources of Dinvolucrata, but also laid a foundation for the research on molecular mechanism of plant acclimation to cold stress.
JI HongChun, SU ZhiXian, YANG Jun, BIAN ChunXiang, HU JinYao, QI Gang . Cloning and Expression of a Coldinduced Gene (DiRCI) from Davidia involucrata (Davidiaceae)[J].Plant Diversity, 2010, 32(02): 151-157.
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks