Plant Diversity ›› 2010, Vol. 32 ›› Issue (02): 151-157.DOI: 10.3724/SP.J.1143.2010.09190

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Cloning and Expression of a Coldinduced Gene (DiRCI) from Davidia involucrata (Davidiaceae)

JI HongChun1,2, SU ZhiXian1,2, YANG Jun1, BIAN ChunXiang1,
HU JinYao2, QI Gang 2

  1. 1 College of Life Science, China West Normal University, Nanchong 637002, China; 2 College of Life Science & Biotechnology, Mianyang Normal University, Mianyang 621000, China
  • Received:2009-09-28 Online:2010-04-25 Published:2010-06-29
  • Contact: SU ZhiXian

Abstract: A coldinduced plasma membrane protein which plays a protective role in avoiding freezing injury for some plants is one of hydrophobic proteins. A gene, named as DiRCI, was isolated from the cDNA library of Davidia involucrata, containing an open reading frame of 174 bp flanked by a 92 bp 5′untranslated sequence and a long 273 bp 3′noncoding region. Alignment analysis indicated that the deduced amino acid sequence of 57 amino acids was high conserved with the MpRCI from Plantago asiatica, and the identity was 895%. Semiquantitative reverse transcriptionpolymerase chain reaction (RTPCR) analysis showed that DiRCI wasn′t detected in various organs of Dinvolucrata above 25℃. When the plant grew under 8℃, the gene expressed in leaves, leafstalk and seeds, but not the roots. The expression level gradually increased after 4 h of the cold treatment and reached a maximum during 24-48 h, and then decreased. The results indicated the gene did have a relation with cold stress in plant and was predicted to be one DiRCI gene. The present data not only enrich gene resources of Dinvolucrata, but also laid a foundation for the research on molecular mechanism of plant acclimation to cold stress.

Key words: Davidia involucrata

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