Plant Diversity ›› 2011, Vol. 33 ›› Issue (3): 275-286.DOI: 10.3724/SP.J.1143.2011.10197

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Role of Protein Kinases in Abscisic Acid and H2O2 Induced Antioxidant Defense in Maize

 XU  Shu-Cheng-1, ZHU  Xue-Lan-2, ZHANG  Li-3   

  1. 1 School of Life Sciences, Fuyang Teachers College, Fuyang 236041, China;
    2 Hongqi Middle School, Fuyang 236034, China; 3 Nanxi Middle School, Lu′an 237321, China
  • Received:2010-11-06 Online:2011-06-25 Published:2011-02-21


Protein phosphorylation plays a central role in mediating abscisic acid (ABA) signaling transduction in plant cells, whereas many of the sensory proteins involving in ABA signaling pathway remain unclear. Here, using a modified in vitro kinase assay, our results showed that ABA and H2O2 induced a rapid activation of total protein kinases and calcium dependent protein kinases in the leaves of maize seedlings. However, ABA-induced activation of protein kinases was inhibited by reactive oxygen species (ROS) inhibitors or scavengers. Protein kinase inhibitors decelerated not only the ABA and H2O2 -induced kinase activity but also ABA or H2O2-induced antioxidant enzyme activity. Protein phosphorylation caused by ABA and H2O2 preceded ABA or H2O2 -induced antioxidant defense obviously. Using in-gel kinase assays, our results showed that several protein kinases with molecular masses of 66kDa, 52kDa, 49kDa and 35kDa respectively might mediate ABA and H2O2-induced antioxidant defense. And the 66kDa and 49kDa protein kinases may act downstream of ROS, and the 52kDa and 35kDa protein kinases may act between ABA and ROS in ABA-induced antioxidant defensive signaling.

Key words: Abscisic acid, Protein kinase, Phosphorylation, Reactive oxygen species, Antioxidant defense

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