Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 761-768.doi: 10.7677/ynzwyj201313192

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Applications and Advances of DNA Barcoding in Ecological Studies

 LUO  Ya-Huang-1、2、3、4, LIU  Jie-1, GAO  Lian-Ming-1**, LI  De-Zhu-1、2   

  1. 1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming650201, China; 2 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy
    of Sciences, Kunming 650201, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China;4 School of Life Sciences, Yunnan University, Kunming 650091, China
  • Received:2013-09-22 Online:2013-11-25 Published:2013-09-30
  • Supported by:

    科技部重大研究计划项目 (2014CB954100);科技基础性工作专项项目 (2012FY110800, 2013FY112600);中国科学院大科学装置项目 (2009-LSFGBOWS-01)

Abstract:

DNA barcoding is a technique used to identify species, which is now widely employed in different biological disciplines. With the development and modification of DNA barcoding, it has become a useful tool for ecological studies. Here, we review the uses of DNA barcoding for quick species identification and/or new and cryptic species discovery, in community phylogenetic reconstruction and ecological forensics, and in interaction networks among species within a community. The techniques of DNA metabarcoding and environmental DNA metabarcoding used in ecological research such as diet analysis and biodiversity assessment are also examined. Finally, we discuss the future prospects of DNA barcoding in ecological research following the development of nextgeneration sequencing technologies, improvement of Largescale Scientific Facilities, and use of new models and software for data analysis.

Key words:  Community phylogeny, Environmental DNA barcoding, DNA metabarcoding, Biodiversity, Next generation sequencing

CLC Number: 

  • Q 948