Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 743-750.doi: 10.7677/ynzwyj201313199

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Selection of Potential Plastid DNA Barcodes for Bambusoideae (Poaceae)

 ZHANG  Yu-Xiao-1、2, HU  Yu-Xing-4, MA  Peng-Fei-1、2, ZHANG  Li-Na-1、2、3, LI  De-Zhu-1、2   

  1. 1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy
    of Sciences, Kunming 650201, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China;
    4 College of Life Sciences, Northwest A & F University, Yangling 712100, China
  • Received:2013-09-30 Online:2013-11-25 Published:2013-10-14

Abstract:

 Bamboos are a taxonomically difficult group. DNA barcoding provides another supplementary method for the bamboo identification. The core DNA barcodes (rbcL+matK) have quite low discriminatory power in bamboos, therefore, it is crucial to select effective DNA barcodes for bamboos. We scanned the entire chloroplast genomes that were published in Bambusoideae in order to search highly variable regions. Sixteen regions were selected including one coding region and 15 intergenic spacers, and the universality, sequencing success rates, and the variable characters were testified in 30 taxa of 22 species in 10 genera of Arundinarieae and Bambuseae. The results indicated that (1) the amplification success rates were high for each region, but there were some problems in sequencing for some regions due to polystructures; (2) indels should be coded as informative characters; (3) the region trnGtrnT(t) was the most variable one among the 16 regions and it was suggested as the prior candidate DNA barcode in Bambusoideae; (4) the combination “trnGtrnT(t)+X” can be used in DNA barcoding and molecularphylogeny reconstruction study of bamboos.

Key words:  Bambusoideae, DNA barcoding, Indels, Molecular phylogeny, Plastid region selection

CLC Number: 

  • Q 948