Relationships within the Lonicera macrantha Complex Based on Morphological and Molecular Data

doi:10.7677/ynzwyj201413094

Abstract

Abstract:

Being one of the most complicated groups in Lonicera (Caprifoliaceae) and distributed mainly in southern China, the Lonicera macrantha complex comprises five species, ie. Lmacrantha, Lmacranthoides, Lsimilis, Lhypoglauca and Lferruginea. The complex is also found to be the main substitute of Ljaponica for Jin Yin Hua in southern China. To clarify the relationships within the complex, investigation of morphology and habitat of each species, observation of microfeatures of leaf epidermis, and molecular phylogenetic analysis were carried out. The results show that only L. macranthoides can be easily distinguished by phylogenetic methods, and that the complex can be roughly divided into three groups based on morphological characters: Lhypoglauca is distinct in its mushroomshaped glands and long trichomes on leaf epidermis; Lferruginea is close to Lmacrantha in the thin trichomes, but they differ in length of corolla tube and stomatal structure; Lmacranthoides is morphologically similar to Lsimilis in the dense trichomes, but they can be distinguished by characters of cuticular membrane. Further studies on resolving the speciation and species dispersal within the complex are still needed.

Key words: Lonicera, Species complex, Epidermis, Molecular phylogeny, Taxonomy

CLC Number:

Q 949

DONG Hong-Jin, PENG Hua. Relationships within the Lonicera macrantha Complex Based on Morphological and Molecular Data[J]. Plant Diversity, 2014, 36(02): 133-141.

References

Angiosperm Phylogeny Group, 1998. An ordinal classification for the families of flowering plants［J］. Annals of the Missouri Botanical Garden, 85 (4): 531—553
Ascensao L, Natalia M, Salqme PM, 1997. Peltate glandular trichomes of Leonotis leonurus leaves: ultrastructure and histochemical characterization of secretions［J］. International Journal of Plant Science, 158: 249—258
Bremer B, Bremer K, Chase M et al., 2009. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III［J］. Botanical Journal of the Linnean Society, 161: 105—121
Donoghue MJ, Olmstead RG, Smith JF et al., 1992. Phylogenetic relationships of Dipsacales based on rbcL sequences［J］. Annals of the Missouri Botanical Garden, 333—345
Donoghue MJ, Bell CD, Li J, 2001a. Phylogenetic patterns in Northern Hemisphere plant geography［J］. International Journal of Plant Sciences, 162 (6): 41—52
Donoghue MJ, Eriksson T, Reeves PA et al., 2001b. Phylogeny and phylogenetic taxonomy of Dipsacales, with special reference to Sinadoxa and Tetradoxa (Adoxaceae) ［J］. Harvard Papers in Botany, 6 (2): 459—480
Donoghue MJ, Bell CD, Winkworth RC, 2003. The evolution of reproductive characters in Dipsacales［J］. International Journal of Plant Sciences, 164 (5): 453—464
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］. Phytochemical Bulletin, 19: 11—15
Hamilton MB, 1999. Four primer pairs for the amplification of chloroplast intergenic regions with intraspecific variation. Molecular Ecology, 8: 521—523
Howarth DG, Martins T, Chimney E et al., 2011. Diversification of CYCLOIDEA expression in the evolution of bilateral flower symmetry in Caprifoliaceae and Lonicera (Dipsacales) ［J］. Annals of Botany, 107 (9): 1521—1532
Huang LH (黄丽华), Chen X (陈训), 2005. Scanning electron microscopic observation of leaf epidermis from eight medical plants of Lonicera genus［J］. Guizhou Science (贵州科学), 23 (1): 54—57
Jacobs B, Pyck N, Smets E, 2010. Phylogeny of the Linnaea clade: Are Abelia and Zabelia closely related?［J］. Molecular Phylogenetics and Evolution, 57 (2): 741—752
Judd WS, Campbell CS, Kellog EA et al., 2007. Plant Systematics: a Phylogenetic Approach, 3 ed［M］. Sunderland, MA: Sinauer Associates
Kim YD, Kim SH, 1999. Phylogeny of Weigela and Diervilla (Caprifoliaceae) based on nuclear rDNA ITS sequences: biogeographic and taxonomic implications［J］. Journal of Plant Research, 112 (3): 331—341
Landrein S, Prenner G, Chase MW et al., 2012. Abelia and relatives: phylogenetics of Linnaeeae (DipsacalesCaprifoliaceae s.l.) and a new interpretation of their inflorescence morphology［J］. Botanical Journal of the Linnean Society, 169 (4): 692—713
Li Q (李强), Deng Y (邓艳), Yu LJ (余龙江) et al., 2007. Research on structure of leaf epidermis of two species of Lonicera by SEM and its ecological adaption［J］. Guihaia (广西植物), 27 (2): 146—151
Mabberley DJ, 2008. Mabberley’s Plant Book: a Portable Dictionary of Plants, Their Classifications, and Uses, 3rd edition［M］. Cambridge: Cambridge University Press
Posada D, Crandall KA, 1998. Modeltest: testing the model of DNA substitution［J］. Bioinformatics, 14 (9): 817—818
Pyck N, Roels P, Smets E, 1999. Tribal relationships in Caprifoliaceae: evidence from a cladistic analysis using ndhF sequences［J］. Systematics and Geography of Plants, 69 (2): 145—159
Rehder A, 1903. Synopsis of the genus Lonicera［J］. Missouri Botani
cal Garden Annual Report, 14: 27—232
Ronquist F, Huelsenbeck JP, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models［J］. Bioinformatics, 19 (12): 1572—1574
Shaw J, Lickey EB, Schilling EE et al., 2007. Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: the tortoise and the hare III［J］. American Journal of Botany, 94: 275—288
Soltis DE, Soltis PS, 1998. Choosing an approach and an appropriate gene for phylogenetic analysis［A］. In: Soltis DE, Soltis PS, Dpyle JJ (eds.), Molecular Systematics of Plants II: DNA Sequencing［M］. Boston: Kluwer, 1—42
Simmons MP, Ochoterena H, 2000. Gaps as characters in sequencebased phylogenetic analyses［J］. Systematic Biology, 49: 369—381
Smith SA, 2009. Taking into account phylogenetic and divergencetime uncertainty in a parametric biogeographical analysis of the Northern Hemisphere plant clade Caprifolieae［J］. Journal of Biogeography, 36 (12): 2324—2337
Sun Z, Gao T, Yao H et al., 2011. Identification of Lonicera japonica and its related species using the DNA barcoding method［J］. Planta Medica, 77 (3): 301—306
Swofford DL, 2002. PAUP*40: phylogenetic analysis using parsimony (* and other methods) ［CP］. Sunderland, Massachusetts: Sinauer Associates
Taberlet PL, Gielly L, Pauton G et al., 1991. Universal primers for amplification of three noncoding regions of chloroplast DNAn［J］. Plant Molecular Biology, 17: 1105—1109
Tamura K, Peterson D, Peterson N et al., 2011. MEGA5: Molecular evolutionary genetics analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony methods［J］. Molecular Biology and Evolution 28: 2731—2739
Tank DC, Donoghue MJ, 2010. Phylogeny and phylogenetic nomenclature of the Campanulidae based on an expanded sample of genes and taxa［J］. Systematic Botany, 35 (2): 425—441
Theis N, Donoghue MJ, Li JH, 2008. Phylogenetics of the Caprifolieae and Lonicera (Dipsacales) based on nuclear and chloroplast DNA sequences［J］. Systematic Botany, 33 (4): 776—783
Whittall JB, MedinaMarino A, Zimmer EA et al., 2006. Generating singlecopy nuclear gene data for a recent adaptive radiation［J］. Molecular Phylogenetics and Evolution 39: 124—134
Yang QE, Landrein S, Osborne J et al., 2011. Caprifoliaceae［A］. In: Wu ZY, Raven PH (eds.), Flora of China［M］. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press, 19: 616—641
Zhang WH, Chen ZD, Li JH et al., 2003. Phylogeny of the Dipsacales sl. based on chloroplast trnLF and ndhF sequences［J］. Molecular Phylogenetics and Evolution, 26 (2): 176—189Appendix: Voucher and locality of species sampled in this study (all deposited in KUN)
No.TaxaLocalityVoucher1-4Lonicera hypoglaucaLonglin, GuangxiDong HJ. & Yin ZJ. 2115-8LhypoglaucaNanchuang, ChongqingDong HJ. & Tang Y. 2849-10LhypoglaucaLonglin, GuangxiDong HJ. & Yin ZJ. 21011-15LhypoglaucaLechang, GuangdongDong HJ. & Yin ZJ. 18016-17LhypoglaucaQuanzhou, GuangxiDong HJ. & Yin ZJ. 20518-21LhypoglaucaRenhua, GuangdongDong HJ. & Yin ZJ. 16822-25LhypoglaucaDayu, JiangxiDong HJ. & Yin ZJ. 15426-27LhypoglaucaChengbu, HunanDong HJ. & Yin ZJ. 18428-29LhypoglaucaChengbu, HunanDong HJ. & Yin ZJ. 18530-31LhypoglaucaLichuang, HubeiDong HJ. & Tang Y. 25932LhypoglaucaJiangkou, GuizhouDong HJ. et al. 93133-35LsimilisEmei, SichuanDong HJ. & Tang Y. 334A36-39LsimilisLichuang, HubeiDong HJ. & Tang Y. 25840-42LsimilisHefeng, HubeiDong HJ. & Tang Y. 28343LsimilisYuanmou, YunnanFang W. 1115344-46LsimilisYuanmou, YunnanXiang CL. et al. 50347-48LsimilisWuding, YunnanXiang CL. et al. 51149-52L. similisJiangkou, GuizhouDong HJ. et al. 65453-56LmacranthaQuanzhou, GuangxiDong HJ. & Yin ZJ. 20457-60LmacranthoidesLechang, GuangdongDong HJ. & Yin ZJ. 17861-64LmacranthoidesQuanzhou, GuangxiDong HJ. & Yin ZJ. 20665-68LferrugineaJingdong, YunnanDong HJ. et al. 83769-72LpampaniniiLechang, GuangdongDong HJ. & Yin ZJ. 17773-77LconfusaGuilin, GuangxiShen XL. sn.78LcalcurataXundian, YunnanDong HJ. et al. 84179LnubiumEmei, SichuanDong HJ. & Tang Y. 334B80LjaponicaTianmushan, ZhejiangDong HJ. & Yin ZJ. 042

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