The Effects of Preservation Methods and Storage Time on Estimating the Nuclear DNA Content (2C-value) of Bamboos

doi:10.7677/ynzwyj201413087

Abstract

Abstract:

Measurement of nuclear DNA content of bamboos (2Cvalue) is important for scientific research. Sample preservations were required for nuclear DNA content estimate using flow cytometry method, when plants tissues were collected from remote areas. Three bamboo species (Dendrocalamus latiflorus, Chimonobambusa tumidissinoda and Acidosasa purpurea) were selected in this study. Samples were treated with two preservation methods and nuclear DNA contents were estimated by flow cytometry after four days, eight days, twelve days and sixteen days, respectively. Coefficient of variation (CV) was an indicator of sample quality which affects the accuracy and precision of DNA content estimates. Differences in nuclear DNA content (2Cvalue) and variations in CVs were analyzed to evaluating the effects of two preservation methods and storage time. Univariate ANOVA analysis revealed that CVs were influenced by storage time significantly (P < 0001). Besides, differences of 2Cvalues between preserved tissues and fresh tissues were influenced by preservation methods significantly (P < 0001). CVs were increasing when time elapsed. After short time preservation, nuclear DNA contents generated from dried tissues were higher than those from fresh tissues, while nuclear DNA contents generated from fixed tissues were lower than those from fresh tissues. We concluded that data quality decreases along with the preservation time increases, expressed as higher CVs. Silica gel desiccant treatment and sample protector treatment could contribute to variations in 2Cvalues of bamboos and the changes of 2Cvalues were lower than 10%.

Key words: Bamboos, Nuclear DNA content, Preserve

CLC Number:

Q 78

ZHANG Yi-Chi, LI Xia, GUO Zhen-Hua. The Effects of Preservation Methods and Storage Time on Estimating the Nuclear DNA Content (2C-value) of Bamboos[J]. Plant Diversity, 2014, 36(02): 227-232.

References

Arumuganathan K, Earle E, 1991. Estimation of nuclear DNA content of plants by flow cytometry［J］. Plant Molecular Biology Reporter, 9: 229—241
Bainard JD, Husband BC, Baldwin SJ et al., 2011. The effects of rapid desiccation on estimates of plant genome size［J］. Chromosome Research, 19: 825—842
Bennett M, Leitch I, 2005. Plant genome size research: a field in focus［J］. Annals of Botany, 95: 1—6
Bennett M, Leitch I, 2011. Nuclear DNA amounts in angiosperms: targets, trends and tomorrow［J］. Annals of Botany, 107: 467—590
DOLEEL J,Barto J,2005.Plant DNA flow cytometry and estimation of nuclear genome size［J］.Annals of Botany,95:99—110
Dolezel J, Greilhuber J, Suda J, 2007. Estimation of nuclear DNA content in plants using flow cytometry［J］. Nature Protocols, 2: 2233—2244
Esteban J, Sheibani K, Owens M et al., 1991. Effects of various fixatives and fixation conditions on DNA ploidy analysis. A need for strict internal DNA standards［J］. American Journal of Clinical Pathology, 95(4): 460
Galbraith DW, 2009. Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values［J］. Cytometry Part A, 75: 692—698
Galbraith DW, Harkins KR, Maddox JM et al., 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues［J］. Science (New York, NY), 220: 1049
Goff SA, Ricke D, Lan TH et al., 2002. A draft sequence of the rice genome (Oryza sativa L. ssp. japonica) ［J］. Science, 296: 92—100
Gong N (弓娜), Tian XM (田新民), Zhou XY (周香艳) , 2011. Applications of flow cytometry in plant research analysis of nuclear DNA content and ploidy level in plant cells［J］. Chinese Agricultural Science Bulletin (中国农学通报), 27: 21—27
Greilhuber J, Temsch EM, Loureiro J, 2007. Nuclear DNA content measurement［J］. Flow Cytometry with Plant Cells: Analysis of Genes, Chromosomes and Genomes, 4: 67—101
Gui YJ, Wang S, Quan LY et al., 2007. Genome size and sequence composition of moso bamboo: a comparative study［J］. Science in China Series C: Life Sciences, 50: 700—705
Janzen DH, 1976. Why bamboos wait so long to flower［J］. Annual Review of Ecology and Systematics, 7: 347—391
Kumar PP, Turner IM, Nagaraja Rao A et al., 2011. Estimation of nuclear DNA content of various bamboo and rattan species［J］. Plant Biotechnology Reports, 5: 317—322
Li DZ, Wang ZP, Stapleton CMA et al., 2006. Poaceae［A］. In: Wu ZY, Raven PH (eds.), Flora of China, Vol 22［M］. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press, 103—105
Li LB (李潞滨), Wu JY (武静宇), Hu T (胡陶) et al., 2008. Estimation of genome size of moso bamboo (Phyllostachys edulis) ［J］.Chinese Bulletin of Botany(植物学通报),25:574—578
Loureiro J, Trávnícˇek P, Rauchová J et al., 2010. The use of flow cytometry in the biosystematics, ecology and population biology of homoploid plants［J］. Preslia, 82: 3—21
Suda J, Trávnícˇek P, 2006. Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry—new prospects for plant research［J］. Cytometry Part A, 69: 273—280
Swift H, 1950. The constancy of desoxyribose nucleic acid in plant nuclei［J］. Proceedings of the National Academy of Sciences of the United States of America, 36: 643

[1]	Yu-Xiao Zhang, Cen Guo, De-Zhu Li. A new subtribal classification of Arundinarieae (Poaceae, Bambusoideae) with the description of a new genus [J]. Plant Diversity, 2020, 42(03): 127-134.
[2]	YE Lin-Jiang- , ZHANG Zhi-Rong-, SUN Zhi-Xia-, TIAN Shuang. The Determination of Nuclear DNA Content (2Cvalue) on Some Representative Genus and Species of Magnoliaceae [J]. Plant Diversity, 2015, 37(05): 605-610.
[3]	CHEN Zong- Lian,HOU Sui- Wen, YU Hong- Yuan. Tissue Culture of Pyrethrum cinerariifolium [J]. Plant Diversity, 1998, 20(03): 1-3.

The Effects of Preservation Methods and Storage Time on Estimating the Nuclear DNA Content (2C-value) of Bamboos

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics