Plant Diversity ›› 2011, Vol. 33 ›› Issue (5): 529-534.DOI: 10.3724/SP.J.1143.2011.11064

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Development of EST-SSR Markers from Jatropha curcas (Euphorbiaceae) and Their Application in Genetic Diversity Analysis among Germplasms

 YANG  Chun-1、2, LIU  Ai-Zhong-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China;
    2  Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-04-18 Online:2011-10-25 Published:2011-05-19
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Jatropha curcas (Euphorbiaceae) has created tremendous interest all over the world for the use of its seed oil as a commercial source of biodiesel. Based on 9843 ESTs available from the developing seeds of Jatropha curcas, we identified 1009 SSRs in 4640 unigenes and developed 11 polymorphic EST-SSR markers which exhibited a low level of genetic diversity among germplasms, i.e. allele number varied from 2 to 3, with a average of 2.45; Heterozygosity (He) ranged from 0.0887-0.5128, with a average of 0.2736; Polymorphic Information Content (PIC) ranged from 0.0847-0.4031, with a average of 0.2313. Further, we analyzed the genetic relationships among 24 germplasms collected from different areas in southern China, northern Vietnam, and India using the 11 EST-SSR markers. The results showed that there was no a geographic pattern of genotypes across the collection areas of Jatropha curcas. The EST-SSR markers developed in current study is useful for both genetic diversity analysis and identification of genetic relationships among germplasms in Jatropha curcas.

Key words: Biodiesel plant, EST-SSR, Genetic diversity, Jatropha curcas

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