Plant Diversity ›› 2015, Vol. 37 ›› Issue (03): 301-308.doi: 10.7677/ynzwyj201514127

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The Relationship between Aleuritopteris humatifolia and A-chrysophylla Based on Three Chloroplast DNA Sequences Analyses

 WANG  Wan-1, YANG  Wen-Li-2, DOU  Ping-1, ZHAO  Dan-Dan-1, ZHANG  Gang-Min-1   

  1. 1College of Nature Conservation Beijing Forestry University, Beijing 100083, China; 2 College of Landscape
    Architecture and Tourism, Hebei Agricultural University, Baoding, Hebei 071000, China
  • Received:2014-09-15 Online:2015-05-25 Published:2015-02-03
  • Supported by:

    国家自然科学基金 (31270253);国家基金委重大国际合作项目 (31110103911)

Abstract:

Total DNA from 27 species including Aleuritopteris humatifolia and Achrysophylla and related taxa was extracted in this research, and the rbcL, trnLF and matK sequences were amplified and sequenced. The sequences were aligned by ClustalX. The phylogenetic trees were constructed by Maximum parsimony methods and the intraspecific and interspecific genetic distance were computed in MEGA 60. The results showed as follows: no significant difference among rbcL, trnLF and matK sequences of one sample of Ahumatifolia and two samples of Achrysophy
lla, and the maximum genetic distance between Ahumatifolia and Achrysophylla were less than the minimum genetic distance between Ahumatifolia and other closely related species. Therefore, our study support the taxonomic treatment of reducing Ahumatifolia to synonym of Achrysophylla based on our phylogenetic analysis combining the evidences from their morphological characters of blades, spores, indusia, and rhizome scales.

Key words: Aleuritopteris humatifolia, A-chrysophylla, cheilanthoid ferns, rbcL, trnL-F, matK

CLC Number: 

  • Q 75, Q 949