Plant Diversity ›› 2006, Vol. 28 ›› Issue (06): 649-652.

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Tissue Culture and Plant Regeneration of Jatropha curcas (Euphorbiaceae)

QIN Hong; SONG Song-Quan; Long Chun-Lin; CHENG Hong-Yan   

  1. Xishuangbanna Tropical Botanical Garden , Chinese Academy of Sciences
  • Received:2006-07-12 Online:2006-12-25 Published:2006-12-25
  • Contact: SONG Song-Quan

Abstract: In this paper , plumules, cotyledons, hypocotyls, blades, petioles and stalks of physic nut ( Jatropha curcas L. ) were used as explants, and callus induction and plant regeneration were studied on MS medium contained different concentrations of 6-BA and NAA. The results showed that the MS medium with 5.0 mg/L BA and 1.0 mg/L NAA was the best for callus induction, and with 5.0mg/L BA and 0.1mg/L NAA, for formation of adventitious bud, and with 1.0mg/L BA and 1.0 mg/L NAA, for bud growth, and that 1􊄯2 MS medium with 1.0 mg/L NAA was the best for formation and growth of adventitious root .

Key words: Callus