Plant Diversity ›› 2014, Vol. 36 ›› Issue (04): 505-512.doi: 10.7677/ynzwyj201413181

• Articles • Previous Articles     Next Articles

Cryopreservation of Jiangxi Yanshan Red Bud Taro (Colocasia esculenta var. cormosus cv. Hongyayu) Embryogenic Calli by Encapsulationdehydration

 HONG  Sen-Rong, YIN  Ming-Hua   

  1. College of Life Sciences, Shangrao Normal University, Shangrao 334001, China
  • Received:2013-09-10 Online:2014-07-25 Published:2013-11-01
  • Supported by:

    江西省科技厅2012年农业科技支撑项目基金 (20122BBF60126);2013年度江西省高等学校科技落地计划项目 (KJLD13088)

Abstract:

Cryopreservation of Jiangxi Yanshan red bud taro (Colocasia esculenta var. cormosus cv. Hongyayu) embryogenic calli by encapsulationdehydration was studied. The results showed that the optimal preculture condition of cryopreservation by encapsulationdehydration was precultured on MS medium supplemented with 075mol·L-1 sucrose for 3 days. The optimal dehydration method was dehydration by sterile air in a laminar flow hood for 7h or sterile dry silica gel for 11h. the optimal thawing temperature was 37℃ (2min). The optimal culture condition after cryopreservation was first in the dark for 7d and then transferred to the photoperiod of 14h·d-1. The average survival rate of embryogenic calli after cryopreservation by encapsulationdehydration was about 45%. Cryopreservation time and whether the removal of encapsulated calcium alginate had no significant impact on the survival rate. Morphological and cytological study demonstrated that the regenerants were genetically and morphological stable.

Key words: Red bud taro (Colocasia esculenta var. cormosus cv. Hongyayu), Embryogenic callus, Encapsulation-dehydration, Cryopreservation

CLC Number: 

  • Q 9431