Cryopreservation of Jiangxi Yanshan Red Bud Taro (Colocasia esculenta var. cormosus cv. Hongyayu) Embryogenic Calli by Encapsulationdehydration

doi:10.7677/ynzwyj201413181

Abstract

Abstract:

Cryopreservation of Jiangxi Yanshan red bud taro (Colocasia esculenta var. cormosus cv. Hongyayu) embryogenic calli by encapsulationdehydration was studied. The results showed that the optimal preculture condition of cryopreservation by encapsulationdehydration was precultured on MS medium supplemented with 075mol·L-1 sucrose for 3 days. The optimal dehydration method was dehydration by sterile air in a laminar flow hood for 7h or sterile dry silica gel for 11h. the optimal thawing temperature was 37℃ (2min). The optimal culture condition after cryopreservation was first in the dark for 7d and then transferred to the photoperiod of 14h·d-1. The average survival rate of embryogenic calli after cryopreservation by encapsulationdehydration was about 45%. Cryopreservation time and whether the removal of encapsulated calcium alginate had no significant impact on the survival rate. Morphological and cytological study demonstrated that the regenerants were genetically and morphological stable.

Key words: Red bud taro (Colocasia esculenta var. cormosus cv. Hongyayu), Embryogenic callus, Encapsulation-dehydration, Cryopreservation

CLC Number:

HONG Sen-Rong, YIN Ming-Hua. Cryopreservation of Jiangxi Yanshan Red Bud Taro (Colocasia esculenta var. cormosus cv. Hongyayu) Embryogenic Calli by Encapsulationdehydration[J]. Plant Diversity, 2014, 36(04): 505-512.

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