Plant Diversity ›› 2006, Vol. 28 ›› Issue (2): 108-114.

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The Application of Four DNA Sequences to Studying Molecular Phylogeny of Camellia ( Theaceae) *

 YANG  Jun-Bo-1、2,   Li-Hong-Tao-1,   Yang-Shi-Xiong-1,   Li-De-Zhu-1**   

  1. 1 Laboratory of Bi odiversity and Bi ogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;
    2 Graduate School of the Chinese Academy of Sciences , Beijing 100039, China
  • Online:2006-04-25 Published:2014-01-17


Four DNA sequences) ITS, waxy , trnL- F, and rpL 16 have been used to resolve the inter- species relationships
of twenty- one species representing 10 out of the 14 sections in Camellia. The results indicated that: ( 1) PCR amplification
and sequencing were easy for trnL- F and rpL16 sequences, however, the divergence rates of the two chloroplast
sequences are too slow with few phylogenetically informative sites in data matrix es ( 9 sites in trnL- F and 20 sites in
rpL 16) , so they could not provide enough phylogenetic information; ( 2) The PCR products of ITS were easily obtained,
but there were still many problems in sequencing; ( 3) The waxy gene, another sequence from the nuclear genome, has
significant values in resolving the infrageneric relationships in Camellia. The divergence rate is faster than aforementioned
sequences. There were more informative sites ( 92) in data matrix though PCR amplification was difficult because it is affected
by quantity and quality of template DNA and it is a single copy gene in Camellia. The present study indicated that
the waxy gene had great potential values in the systematic study of Camellia while other three sequences are unfitted to resolve
the problem. The results of our DNA analysis of three segments support that the genus Camellia is a monophyletic
group, but the inter- species relationships is still needed more study.

Key words: Camellia, DNA sequences, Divergence rate, Molecular phylogeny

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