Plant Diversity ›› 2008, Vol. 30 ›› Issue (6): 688-692.DOI: 10.3724 SP.J.1143.2008.08038

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Molecular Cloning, Expression and Activity Analysis of a Glutathione S-transferase in Rice

HU Ting-Zhang1 ,2 , HUANG Xiao-Yun2 , XIAO Guo-Sheng2 , QV Xiao-Xiao1   

  1. 1 Bioengineering College of Chongqing University , Chongqing 400044 , China; 2 Department of Biology, Chongqing Three Gorges University , Chongqing 404000 , China

  • Received:2008-03-15 Online:2008-12-25 Published:2008-12-25

Abstract: OsGSTL1 is a glutathione S-transferase (GSTs , EC 2. 5 . 1 . 18 ) , lambda class gene in chromosome 3 of rice ( Oryza sativa L .) . It includes 8 introns and 9 exons, and encodes a protein of 243 amino acid residues . The OsGSTL1 gene was cloned into pYTV vector and was transformed into yeast strain PEP4 .Western blot analysis showed the exogenous OsGSTL1 was expressed in transformed yeast . GST activity of crude extracts of yeast showed the OsGSTL1 transgenic yeast had higher levels of GST activities than control yeasts, which demonstrate the OsGSTL1 has glutathione S- transferase activity .

Key words: Glutathione S-transferase

CLC Number: