Plant Diversity ›› 2011, Vol. 33 ›› Issue (3): 330-334.DOI: 10.3724/SP.J.1143.2011.11021

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The Clone and Analysis on Different Lengths of 3′ Transcription of BcMF13 from Brassica rapa ssp. chinensis (Cruciferae)

 NIE  Chuan-Peng, LI  Yan-Yan   

  1. School of Life Sciences, Fuyang Teachers College, Fuyang 236041, China
  • Received:2011-01-30 Online:2011-06-25 Published:2011-03-10


Two different lengths of 3′ transcripts were got in cloning the pollen development gene BcMF13, using RACE technology. Compared with the EST database and found that their high sequence similarity are derived from cruciferous Brassica, Raphanus, plants’ reproductive organs, and different transcripts were also found in these sources. The existence of these different transcripts exist that BcMF13 shear isomers, flexible and complex regulation of the process, reflecting the important function of the gene. Expression analysis of RT-PCR have shown that BcMF13 only expressed in fertile lines, no in sterile lines, and mainly concentrated in the large flower buds and stamen. Analysis of the deduced protein structure of BcMF13 found that the proteins encoded multiple biological active sites. These results show that BcMF13 was late expression gene on pollen fertility. BcMF13 was active in the implementation of physiological function.

Key words: Brassica campestris Male Fertility 13 (BcMF13), RACE, Different lengths of 3&prime, transcription

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