DNA Barcoding of Edible Boletes (Boletaceae) from Yunnan, China

doi:10.7677/ynzwyj201313201

Abstract

Abstract:

iFlora is designed to integrate traditional taxonomy knowledge with DNA sequencing and biological information. Through a series of key technological innovations and integrations, the main objective of iFlora is to construct the nextgeneration Flora or intelligent Flora, which should fulfill the function of accurately and rapidly identifying species and acquiring related digital information. The most important and urgent task for iFlora development is to search for the standard DNA barcode to distinguish most of the plants and economically important mushrooms. To establish a standard DNA barcode for the edible boletes in Yunnan, samples of common boletes in the free markets regarded as four “species” by local mushroom hunters were collected and studied. Our date indicated that these four “species” in fact represent 12 distinct species. Furthermore, four candidate markers, the large subunit nuclear ribosomal RNA (nrLSU), the translation elongation factor 1alpha (tef1α), the RNA polymerase II largest subunit (rpb1) and the RNA polymerase II second largest subunit (rpb2) were tested using the eukaryotic general primers for their feasibility as barcodes of the species of Boletus. This study indicates that the four candidate markers are of very high PCR amplification and sequencing success rate (100% and 100% respectively), and there are no overlapping between intra and inter specific variation. All four candidate markers showed high species resolution. Compared with nrLSU, rpb1, tef1α and rpb2 had much more clearly defined barcoding gaps. This study showed that rpb1 can be used as a primary barcode marker considering that rpb1 showed high sequence variation among species and low variation within species, while tef1α and rpb2 can be proposed as supplementary barcodes for the boletes.

Key words: iFlora, DNA barcoding, Boletes, Species recognition, Edible mushrooms

CLC Number:

LI Yan-Chun, WU Gang, YANG Zhu-Liang. DNA Barcoding of Edible Boletes (Boletaceae) from Yunnan, China[J]. Plant Diversity, 2013, 35(6): 725-732.

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