Asymbiotic Seed Germination and in vitro Seedling Rapid Development of Paphiopedilum spicerianum

doi:10.7677/ynzwyj201515012

Abstract

Abstract:

In this paper, we studied the asymbiotic seed germination and seedling development of Paphiopedilum spicerianum, a wild plant with extremely small populations (PSESP) in China. By analogizing the influences of different media, seed maturity, pretreatments and lights on the seed germination, the results showed that the best germination condition is using the seeds at 270 days after pollination, pretreating with 1% NaOCl for 40 min, on the 1/4MS+10% coconut water medium under 24 hours darkness for 4 weeks. For seedling formation, 30g·L-1 Hyponex No 1 medium with 10mg·L-1 αnaphthlene acetic acid, 05g·L-1 activated charcoal and 10% banana homogenate was the most effective. For seedling development, the same medium used for seedling formation with supplemented 10g·L-1 6benzyladenine was most suitable.

Key words: Paphiopedilum spicerianum, Asymbiotic seed germination, Wild Plants with Extremely Small Populations, Orchid conservation, in vitro seedling rapid production

CLC Number:

Q 945

CHEN Ying, FAN Xu-Li, GAO Jiang-Yun. Asymbiotic Seed Germination and in vitro Seedling Rapid Development of Paphiopedilum spicerianum[J]. Plant Diversity, 2015, 37(05): 611-615.

References

Abraham S, Augustine J, Thomas TD, 2012. Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats［J］. Physiologicaland Molecular Biology of Plants, 18 (3): 245—251
Arditti J, 1967. Factors affecting the germination of orchid seeds［J］. Botanical Review, (33): 1—97
Arditti J, 1982. Orchid Biology, Reviews and Perspective Ⅱ［M］. Ithaca, NY: Cornell University Press, 352
Arditti J, Ernst R, 1993. Micropropagation of Orchids［M］. New York: John Wiley and Sons
Bektas E, Cuce M, Sokmen A, 2013. In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey［J］. Turkish Journal of Botany, (37): 336—342
Chen ZL (陈之林), Ye XL (叶秀粦), Liang CY (梁承邺), 2004. Seed germination in vitro of Paphiopedilum armeniacum and Pmicranthum［J］. Acta Horticulturae Sinica (园艺学报), 31 (4): 540—542
Dutra D, Kane ME, Richardson L, 2009. Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum: a propagation protocol for an endangered Florida native orchid［J］. Plant Cell Tissue and Organ Culture, (96): 235—243
Kauth P, dutra D, Johnson T, 2008. Techniques and applications of in vitro orchid seed germination［A］// Teixeira da Silva JA ed., Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues ［M］. Global Science Books, UK, 375—391
Lee YI, Lu CF, Chung MC et al., 2007. Changes in endogenous abscisic acid levels and asymbiotic seed germination of a terrestrial orchid, Calanthe tricarinata Lindl［J］. Journal of the American Society for Horticulture Science, (132): 246—252
Liu QF (刘其府), Fu YY (傅燕艳), Zeng SJ (曾宋君), 2012. Test on asymbiotic seed germination of Paphiopedilum concolor (Bateman) Pfitz.［J］. Guangdong Agricultural Sciences (广东农业科学), 39 (12): 47—49
Long B, Niemiera A, Cheng Z et al., 2010. In vitro propagation of four threatened Paphiopedilum species (Orchidaceae) ［J］. Plant Cell Tissue and Organ Culture, (101): 151—162
Murashige T, Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture［J］. Physiologia Plantarum, (15): 473—497
Nhut D, Trang P, Vu N et al., 2005. A wounding method and liquid culture in Paphiopedlium delenatii propagation［J］. Propagation of Ornamental Plants, (5): 156—161
Ramsay M, Stewart J, 1998. Reestablishment of the lady′s slipper orchid (Cypripedium calceolus L.) in Britain［J］. Botanical Journal of the Linnean Society, (126): 173—181
Rasmussen HN, 1995. Terrestrial Orchids from Seed to Mycotrophic Plant［M］. New York: Cambridge University Press
Roy AR, Patel RS, Patel VV et al., 2011. Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex. Lindl. (Blue Vanda): An in vitro protocol for an endangered orchid［J］. Scientia Horticulturae, (128): 325—331
Sileshi G, 2012. A critique of current trends in the statistical analysis of seed germination and viability data［J］. Seed Science Research, (22):145—159
Stewart SL, Zettler LW, 2002. Symbiotic germination of three semiaquatic rein orchids (Habenaria repens, Hquinquiseta, Hmacroceratitis) from Florida［J］. Aquatic Botany, (72): 25—35
Suzuki RM, Moreira VC, Pescador R et al., 2012. Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina［J］. In Vitro Cellular Developmental BiologyPlant, (48): 500—511
Wang LH (王莲辉), Jiang YL (姜运力), Yu JY (余金勇) et al., 2009. Tissue culture and rapid propagation of Paphiopedilum dianthum Tang et Wang［J］. Plant Physiology Communications (植物生理学通讯), 45 (9): 887—888
Ye DP (叶德平), Luo YB (罗毅波), 2006. Paphiopedilum spicerianum, a new record of Orchidaceae from China［J］. Acta Phytotaxonomica Sinica (植物分类学报), 44 (4): 471—473
Zeng SJ (曾宋君), Chen ZL (陈之林), Duan J (段俊), 2006. Asepsis sowing and in vitro propagation of Paphiopedilum hirsutissimum Pfitz［J］. Plant Physiology Communications (植物生理学通讯), 42 (2): 247—247
Zeng SJ (曾宋君), Chen ZL (陈之林), Wu KL (吴坤林) et al., 2007. A Resume of Germination and Mericlone of Paphiopedilum［J］. Acta Horticulturae Sinica (园艺学报), 34 (3): 793—796
Zeng SJ, Wu KL, Teixeira SJ et al., 2012. Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid［J］. Scientia Horticulturae, (138): 198—209
Zhang MG (张铭光), Pan RZ (潘瑞炽), Ye QS (叶庆生), 1994. Influence of moisture on leaf growth and photosynthetic rate of Cymbidium sinense［J］. Journal of South China Normal University (华南师范大学学报), (2): 72—75
Zhou L (周丽), Li SK (李松克), Deng KY (邓克云) et al., 2012. Aseptic sowing and tissue culture propagation of Paphiopedilum gratrixianum［J］. Journal of Anhui Agriculture Science (安徽农业科学), 40 (18): 9590—9592

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