Phylogeographical Study on Eriophyton wallichii (Labiatae) from Alpine Scree of QinghaiTibetan Plateau

doi:10.3724/SP.J.1143.2011.11082

Abstract

Abstract:

In this study, we examined the nuclear ribosomal DNA internal transcribed spacer (ITS) sequence variation of Eriophyton wallichii, a perennial endemic to alpine scree of the QinghaiTibetan Plateau. Nineteen haplotypes were detected by analyzing 187 individuals from 20 populations of E.wallichii. Most populations hold the unique haplotype, which showed different population with different haplotype. An analysis of molecular variance (AMOVA) for populations showed that the genetic variation mainly resided among populations (89.54%), the level of differentiation among populations was very high (GST=0.863; NST=0.957) but didn’t show a significant phylogeographical structure. It is different from foregoing other species reported in QinghaiTibetan Plateau that the haplotypes of E.wallichii showed a distribution pattern that almost one population hold one unique haplotype, hardly share same halotype among populations.We hypothesized the unique genetic structure of E.wallichii might result from allopatric fragmentation in the “islands of alpine” due to the extremely isolated alpine scree and rough topography of the region, through the Quaternary climate oscillation dramatically caused population expanding or shrinking.

Key words: QinghaiTibetan Plateau, Alpine scree, Eriophyton wallichii, Phylogeography, ITS

CLC Number:

Q 948

WANG Xiao-Xiong-, YUE Ji-Pei-, SUN Hang-, LI Zhi-Min. Phylogeographical Study on Eriophyton wallichii (Labiatae) from Alpine Scree of QinghaiTibetan Plateau[J]. Plant Diversity, 2011, 33(6): 605-614.

References

刘腾靓, 2009. 西藏沙棘的谱系地理学研究［D］. 兰州: 兰州大学(硕士学位论文)
吴征镒, 朱彦承主编, 1985. 云南植被［M］. 北京: 科学出版社
Bandelt HJ, Forster P, Rohl A. 1999. Medianjoining networks for inferring intraspecific phylogenies［J］. Molecular Biology and Evolution, 16: 37—48
Chen SY, Wu GL, Zhang DJ et al., 2008. Potential refugium on the QinghaiTibet Plateau revealed by the chloroplast DNA phylogeography of the alpine species Metagentiana striata (Gentianaceae) ［J］. Botanical Journal of the Linnean Society, 157: 125—140
Collevatti RG, Rabelo SG, Vieira RF, 2009. Phylogeography and disjunct distribution in Lychnophora ericoides (Asteraceae), an endangered cerrado shrub species［J］. Annals of Botany, 104: 655—664
Comes HP, Kadereit JW, 1998. The effects of Quatenary climatic changes on plant distribution and evolution［J］. Trends in Plant Science, 3: 432—438
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf material［J］. Phytochemical Bulletin, 9: 11—15
Duan YZ (段义忠), Zhang DJ (张得钧), Gao QB (高庆波) et al., 2010. Characteristic of molecular evolution of Sibiraea angustata based on nrDNA ITS and cpDNA trnLF sequence analysis［J］. Bulletin of Botanical Research (植物研究), 30 (2): 146—151
Dynesius M, Jansson R, 2000. Evolutionary consequences of changes in species’ geographical distributions driven by Milankovitch climate oscillations［J］. Proceedings of the National Academy of Sciences, 97: 9115—9120
Excoffier LP, Laval G, Schneider S, 2006. Arlequin 3.01: An Integrated Software Package for Population Genetics Data Analysis［M］. Switzerland: Institute of Zoology, University of Berne
Forster P, Bandelt HJ, Rhl A et al., 2004. Network 4.2.0.1. Software available free at www.fluxusengineering.com. Fluxus Technology Ltda
Fujii N, Senni K, 2006. Phylogeography of Japanese alpine plants: biogeographic importance of alpine region of central Honshu in Japan［J］. Taxon, 55: 43—52
Fu YX, Li WH, 1993. Statistical tests of neutrality of mutations［J］. Genetics, 133: 693—709
Gao LM, Moller M, Zhang XM et al., 2007. High variation and strong phylogeographic pattern among cpDNA haplotypes in Taxus wallichiana (Taxaceae) in China and North Vietnam［J］. Molecular Ecology, 16: 4684—4698
Gao QB, Zhang DJ, Chen SY et al., 2009. Chloroplast DNA phylogeography of Rhodiola alsia (Crassulaceae) in the QinghaiTibet Plateau［J］. Botany, 87 (11): 1077—1088
Hewitt G, 2000. The genetic legacy of the Quaternary ice ages［J］. Nature, 405: 907—913
Ikeda H, Senni KEI, Fujii N et al., 2006. Refugia of Potentilla matsumurae (Rosaceae) located at high mountains in the Japanese archipelago［J］. Molecular Ecology, 15: 3731—3740
Ikeda H, Setoguchi H, 2007. Phylogeography and refugia of the Japanese endemic alpine plant, Phyllodoce nipponica Makino (Ericaceae)［J］. Journal of Biogeography, 34: 169—176
Koch MA, Kiefer C, Ehrich D et al., 2006. Three times out of Asia Minor: the phylogeography of Arabis alpina L. (Brassicaceae)［J］. Molecular Ecology, 15: 825—839
Kropf M, Kandereit JW, Comes HP, 2003. Differential cycles of range contraction and expansion in European high mountain plants during the late Quaternary: insight from Pritzelago alpine (L) D. Kuntze (Brassicaceae) ［J］. Molecular Ecology, 12: 931—949
Li BS (李渤生), Zhang JW (张经纬), Wang JT (王金亭) et al., 1981. A primary study of subnival vegetation in Xizang［J］. Acta Botanical Sinica (植物学报), 23 (2): 132—139
Li C, Shimono A, Shen HH et al., 2010. Phylogeography of Potentilla fruticosa, an alpine shrub on the QinghaiTibetan Plateau［J］. Journal of Plant Ecology, 3: 9—15
Liu LH (刘伦辉), Yu YD (余有德), Zhang JH (张建华), 1984. The division of vertical vegetation zone in Hengduanshan［J］. Acta Botanica Yunnanica (云南植物研究), 6 (2): 205—216
Mani MS, 1980. Introduction: The high altitude environment［A］. In: Mani MS, Giddings LE eds. Ecology of Highlands (Monographiae Biologicae Volume 40)［M］. The Netherlands: Dr W Junk by Publishers The HagueBostonLondon, 1—9
Mantel N, 1967. The detection of disease clustering and a generalized regression approach［J］. Cancer Research, 27 (2): 209—220
Meng LH, Yang R, Abbott Ill et al., 2007. Mitochondrial and chloroplast phylogeography of Picea crassifolia Kom (Pinaceae) in the QinghaiTibetan Plateau and adjacent highlands［J］. Molecular Ecology, 16: 4128—4137
Nei M, 1987. Molecular Evolutionary Genetics［M］. New York: Columbia University Press
Opgenoorth L, Vendramin GG, Mao K et al., 2010. Tree endurance on the Tibetan Plateau marks the world’s highest known tree line of the Last Glacial Maximum［J］. New Phytologist, 185: 332—342
Pons O, Petit RJ, 1996. Measuring and testing genetic differentiation with ordered versus unordered alleles［J］. Genetics, 144: 1237—1245
Qiu YX, Fu CX, Comes HP, 2011. Plant molecular phylogeography in China and adjacent regions: Tracing the genetic imprints of Quaternary climate and environmental change in the world’s most diverse temperate flora［J］. Molecular Phylogenetics and Evolution, 59 (1): 225—244
Raymond M, Rousset F, 1995. An exact test for population differentiation［J］. Evolution, 49: 1280—1283
Rogers AR, Harpending H, 1992. Population growth makes waves in the distribution of pairwise differences［J］. Molecular Biology and Evolution, 9: 552—569
Rozas J, SanchezDeI Barrie JC, Messeguer X et al., 2003. DNASP: DNA polymorphism analyses by the coalescent and other methods［J］. Bioinformatics, 19: 2496—2497
Rutherford S, D’Hondt S, 2000. Early onset and tropical forcing of 100000year Pleistocene glacial cycles［J］. Nature, 408: 72—75
Scheen AC, Bendiksby M, Ryding O et al., 2010. Molecular phylogenetics, character evolution, and suprageneric classification of Lamoideae (Lamiaceae)［J］. Annals of the Missouri Botanical Garden, 97: 191—217
Smouse PE, Long JC, Sokal RR, 1986. Multiple regression and correlation extensions of the Mantel test of matrix correspondence［J］. Systematic Biology, 35 (4): 627—632
Song MS (宋敏舒), Yue JP (乐霁培), Sun H (孙航) et al., 2011. Phylogeographical study on Primula poissonii (Primulaceae) from Hengduan Mountains［J］. Plant Diversity and Resources (植物分类与资源学报), 33 (1): 91—100
Swofford DL, 2003. PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Version 4.0b10［M］. Sunderland, Massachusetts: Sinauer Associates
Taberlet P, Fumagalli L, WustSaucy AG et al., 1998. Comparative phylogeography and postglacial colonization routes in Europe［J］. Molecular Ecology, 7: 453—464
Tajima F, 1989. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism［J］. Genetics, 7: 381—397
Thompson JD, Gibson TJ, Plewniak F et al., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools［J］. Nucleic Acids Research, 25: 4876—4882
Wang FY, Gong X, Hu CM et al., 2008. Phylogeography of an alpine species Primula secundiflora inferred from the chloroplast DNA sequence variation［J］. Journal of Systematics and Evolution, 46 (1): 13—22
Wang LY, Abbott RJ, Zheng W et al., 2009a. History and evolution of alpine plants endemic to the OinghaiTibetan Plateau: Aconitum gymnandrum (Ranunculaceae)［J］. Molecular Ecology, 18: 709—721
Wang LY, Ikeda H, Liu TL et al., 2009b. Repeated range expansion and glacial endurance of Potentilla glabra (Rosaceae) in the QinghaiTibetan Plateau［J］. Journal of Integrative Plant Biology, 51: 698—706
Weir BS, Cockerham CC, 1984. Estimating Fstatistics for the analysis of population structure［J］. Evolution, 38: 1358—1370
White TJ, Bruns T, Lee S et al., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics［A］. In: Innis MA, Gelfand DH, Sninsky JJ et al. eds. PCR Protocols: A Guide to Methods and Application［M］. San Diego, Ca
lifornia: Academic Press, 315—322
Winograd IJ, Landwehr JM, Ludwig KR et al., 1997. Duration and structure of the past four interglaciations［J］. Quaternary Research, 48: 141—154
Wu CY (吴征镒), Li HW (李锡文), 1977. Labiatae［A］. In: Flora Reipublicae Popularis Sinicae［M］. Beijing: Science Press, 65: 1—649
Yang FS, Li YF, Ding X et al., 2008. Extensive population expansion of Pedicularis longiflora (Orobanchaceae) on the OinghaiTibetan Plateau and its correlation with the Quaternary climate change［J］. Molecular Ecology, 17: 5135—5145
Zhang Q, Chiang TY, George M et al., 2005. Phylogeography of the QinghaiTibetan plateau endemic Juniperus przewalskii (Cupressaceae) inferred from chloroplast DNA sequence variation［J］. Molecular Ecology, 14: 3513—3524
Zhang YH, Volis S, Sun H, 2010. Chloroplast phylogeny and phylogeography of Stellera chamaejasme on the QinghaiTibet Plateau and in adjacent regions［J］. Molecular Phylogenetics and Evolution, 57 (3): 1162—1172

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