Isolation and Characterization of Microsatellite Markers for the Chinese Endemic Species Rhododendron spinuliferum (Ericaceae)

Abstract

Abstract:

Rhododendron spinuliferum (Ericaceae) is an ornamental and medicinal plant endemic to southwest China. In order to study hybridization between Rspinuliferum and Rspiciferum, a FIASCO (Fast Isolation by AFLP of Sequences Containing Repeats) method was used to develop microsatellite markers in Rspinuliferum. A total of 28 microsatellite markers were isolated from 100 SSR primer pairs, of which 22 were polymorphic. Polymorphism of the 22 polymorphic loci was assessed separately in 24 individuals collected from two wild populations. The number of alleles per locus ranged from 2 to 5, with an average of 34, while observed (HO) and expected (HE) heterozygosities varied from 0083 to 0792 and from 0153 to 0744, respectively. The same 28 microsatellite markers were also tested in two wild populations (12 individuals from each) of Rspiciferum. Twenty two of the markers were successfully amplified, of which 20 were polymorphic. Estimates of diversity in two natural populations of Rspiciferum based on the 20 polymorphic markers revealed that number of alleles per locus ranged from 2 to 6, with a mean of 32, while observed (HO) and expected (HE) heterozygosities ranged from 0000 to 0833 and from 0117 to 0736, respectively. These newly developed microsatellite markers will be used in future studies of hybridization and the population genetics of Rspinuliferum and its closely related species.

Key words: Microsatellite markers, Rhododendron spinuliferum, Rhododendron spiciferum, FIASCO, Polymorphism

CLC Number:

Q 943

YAN Li-Jun, ZHANG Zhi-Rong, LI De-Zhu, GAO Lian-Ming. Isolation and Characterization of Microsatellite Markers for the Chinese Endemic Species Rhododendron spinuliferum (Ericaceae)[J]. Plant Diversity, 2014, 36(01): 41-46.

References

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Isolation and Characterization of Microsatellite Markers for the Chinese Endemic Species Rhododendron spinuliferum (Ericaceae)

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